马立克氏病病毒囊膜糖蛋白Ⅰ的原核表达及多克隆抗体的制备

Expression of Glycoprotein I Gene of Marek′s Disease Virus and Preparation of Multiclonal Antibodies against Recombinant gI Protein

提取感染MDV 814A3毒株的鸡胚成纤维细胞(CEF)总基因组,利用PCR技术扩增获得gI基因。将鉴定正确的gI基因克隆至pET-32a(+),构建原核表达载体pET-gI。将诱导表达获得的gI重组蛋白免疫新西兰大白兔,产生的抗血清能够与MDV814A3病毒发生特异性反应。本试验所制备的gI重组蛋白和多克隆抗体将在MDV检测、血清学诊断等方面具有重要应用价值。

The total genome was extracted from chicken embryo fibroblast （CEF）infected with MDV 814A3 strain and the gI gene was amplified by PCR. The prokaryotic expression vector pET-gI was constructed through the gI gene cloned into pET-32a（＋）. The gI recombinant protein was obtained by induced with IPTG,and used to immunized rabbit while the obtained multiclonal antibodies were specific to MDV antigen. The gI recombinant protein and multiclonal antibodies prepared would be useful as tools for antigenic analysis and serological diagnose of MDV.