小菘菜游离小孢子培养和植株再生

Isolated microspore culture and plant regeneration in Brassica campestris L. ssp. chinensis(L.)Makino var. communis Tsen et Lee

以7个基因型的小菘菜品种为供试材料,采用游离小孢子培养技术,研究基因型、培养方法、激素及AgNO3对小孢子胚芽诱导率和植株再生的影响。结果表明:基因型是影响小菘菜小孢子胚芽诱导率的最重要的因子;小菘菜小孢子培养初期用170 g.L-1蔗糖处理1 d后,更换培养基能显著提高小孢子的胚芽诱导率;0.05 mg.L-16-BA和0.2 mg.L-1NAA能促进小菘菜小孢子胚的发生;添加0.1 mg.L-1GA3和0.3或0.4 mg.L-1AgNO3可显著提高小菘菜小孢子胚的胚芽诱导率和平均每胚出芽数。

Isolated microspore culture was carried out by seven different varieties of Brassica campestris L. ssp. chinensis （L.）Makino var. communis Tsen et Lee. Effects of genotypes, mediums, hormones and AgNO3 on embryonic induction rate and plant regeneration were studied. The results showed that the genotype was the most important factor on the inducing rate of microspore-derived embryos ; 13% sucrose culture medium after 1 d preliminary culture in 170 g·L^-1 sugar medium could increase the embryonic induction ;0.05 mg· L^-1 6-BA and 0.2 mg·L^-1 NAA could improve the frequency of microspore embryogenesis; the rate of embryonic germination could increase by adding 0.1 mg.L^-1 GA3 and 0.3 or 0.4 mg.L^-1 AgNO3.