离子液体中壳聚糖磷酸吡哆醛席夫碱衍生物的合成与表征

Synthesis and characterization of chitosan phosphopyridoxal Schiff base derivative in ionic liquid

引言壳聚糖是自然界中唯一含有氨基的碱性多糖,具有多样的生物活性、极好的生物相容性、生物可降解性以及无毒性等特性,是一种新兴的生物功能材料[1-3]。磷酸吡哆醛是维生素B6参与多种代谢反应的一种活性形式。磷酸吡哆醛作为一种辅酶参与所有转氨基反应及一些氨基酸的脱羧及脱氢反应。研究表明,磷酸吡哆醛不但可以用于预防治疗高血压、心血管疾病及糖尿病等,还可用于治疗迟发性运动障碍和治疗难治性儿童期癫痫[4-5]。王涛等[6]以壳聚糖微球为载体,然后固定磷酸吡哆醛等制备得到了低密度脂蛋白亲和吸附剂。

5-Phosphate pyridoxal（PPL）is the active coenzyme form of vitamin B6,acting as a coenzyme in a multitude of biochemical processes,therefore chitosan derivatives containing pyridoxal phosphate skeleton will provide an important material for the application of chitosan in the emerging biological function material.Chitosan phosphopyridoxal Schiff base derivative was synthesized by the condensation reaction of chitosan and 5-phosphate pyridoxal in an ionic liquid,1-butyl-3-methylimidazolium chloride（BmimCl）.The product was characterized by Fourier transform infrared（FT-IR）,nuclear magnetic resonance（NMR）,XRD and TGA.The results showed that phosphopyridoxal group was successfully introduced onto the chitosan chain.It was found that degree of substitution of modified chitosan could reach 16.3%.