氟西汀及吡咯烷二硫代氨基甲酸对体外培养星形胶质细胞分泌白细胞介素6的影响

Effect of fluoxetine and pyrrolidine dithiocarbamate on the lipopolysaccharide-induced interleukin 6 re-lease in cultured rat astrocytes

目的通过体外培养纯化星形胶质细胞，观察氟西汀及吡咯烷二硫代氨基甲酸（Pyrrolidine Dithiocarbamate，PDTC）对磷酸脂多糖（1ipopolysaccharide，LPS）诱导星形胶质细胞释放白细胞介素6（inter—leukin6，IL-6）的影响。方法体外分离并纯化的大鼠星形胶质细胞接种到48孔板中，分为对照组、LPS组、氟西汀组和PDTC组。对照组和LPS组常规培养，氟西汀组和PDTC组加入不同浓度的氟西汀和PDTC孵育48h；然后LPS组、氟西汀组和PDTC组给予1μ/ml LPS刺激24h，对照组常规培养；最后用酶联免疫吸附法检测各组上清液IL-6的水平。结果LPS组[（1975．46±171．54）pg／m1]上清液IL-6的含量与对照组[（10633．00±782．15）pg／m1]相比，差异具有高度统计学意义（P〈0．01）；当氟西汀浓度为10μM、20μM和40μM时，IL-6的含量分别为（6198．6±379．4）pg／ml、（4973．6±132．5）pg／ml和（4747．9±473．9）pg／ml，与LPS组相比，IL-6的含量明显降低，差异具有统计学意义（P〈0．05）；当PDTC的浓度为100μM、150μM和200μM时，IL-6的含量分别为（4821．64±180．8）pg／ml、（4735．7±620．0）pg／ml和（3525．9±240．0）pg／ml，较LPS组明显降低，差异具有统计学意义（P〈0．05）。结论LPS可诱导星形胶质细胞分泌IL-6，氟西汀和PDTC在一定浓度范围内可减弱LPS的这一作用。

Objective To investigate the effects of fluoxetine and pyrrolidine dithidarbamate （PDTC） on the lipopolysaccharide （LPS）-induced interleukine-6 （IL-6） release in cultured rat astrocytes. Methods The pu-rified astrocytes were cultured in 48-well tissue cuhure plate and classified into control group, LPS group ,fluoxetine group and PDTC group. Control group and LPS group were cultured as usual, and fluoxetine group and PDTC group were cultured with fluoxetine or PDTC at different concentrations for 48 hours, and then LPS group, Fluoxetine group and PDTC group were incubated with lug/ml LPS for 24 hours. Finally,the levels of IL-6 in the cell super-natant were detected by enzymatic linked immunosorbent assay （ELISA） method. Results The level of IL-6 in LPS group （（ 1975.46 ± 171.54）pg/ml） was significantly higher than that in control group（ （10633 ± 782.15 ） pg/ml） （P 〈 0.01 ）. The levels of IL-6 were （ 6198.6 ± 379.4 ） pg/ml, （ 4973.6 ± 132.5 ） pg/ml and （4747.9 ± 473.9） pg/nd respectively when the concentrations of fluoxetine were 10 μM ,20 μM and 40 μM, and （4821.6 ± 180.8） pg/ml, （4735.7 ± 620. 0） pg/ml and （3525.9 ± 240.0） pg/ml respectively when the concentrations of PDTC were 100 μM, 150 μM and 200 μM. There was significant difference in the levels of IL-6 between LPS group and fluoxetine group （P 〈 0.05 ）, as well as between LPS group and fluoxetine group （P 〈 0.05 ）. Conclusion LPS can induce IL-6 release from astrocytes, while fluoxetine or PDTC at some concentrations can suppress LPS-in-dueed IL-6 release.