紫红笛鲷线粒体全序列与笛鲷属鱼类分化年代的贝叶斯分析

Complete mitochondria sequence of Lutjanus argentimaculatus and Bayesian analysis

利用常规PCR未纯化产物直接测序的方法,获得紫红笛鲷(Lutjanus argentimaculatus)全长为16 543 bp线粒体DNA全序列,GenBank登录号为JN182927。结合GenBank中的已有数据比较分析表明:紫红笛鲷与笛鲷属(Lutjanus)线粒体基因组成基本一致,符合脊椎动物的特征,各基因间进化速率差异显著;13个蛋白编码基因拼接序列贝叶斯建树的后验率显著高于单基因或全序列,更适用于笛鲷属的贝叶斯法系统分化分析和年代推断;13个蛋白编码基因及其拼接序列进行的系统进化分析均支持紫红笛鲷与勒氏笛鲷(Lutjanus russellii)及海鸡母笛鲷(Lutjanus rivulatus)亲缘关系较近;基于13个蛋白编码基因拼接序列的贝叶斯松散分子钟推算出笛鲷属鱼类两个主要支系大约在61.5Ma前古新世的达宁阶与蒙蒂阶交界时期发生分化。

We got 16 543 bp Red snapper （Lutjanus argentimaculatus） complete mitochondrial DNA sequence through common PCR, with the GenBank registration number being JN182927. Our sequence and the data in Gen-Bank, red snapper＇s mitochondrion gene composition were consistent with other snappers, according to the characteristics of vertebrates. Bayesian tree of complete sequence and 13 protein-coding genes and their splicing sequence indicated that： red snapper was close to L. russellii and L. rivulatus. The evolution rate differences of different mi- tochondrial sections were obvious. COX 1, ND 2 and ATPase6 Bayesian posterior rates were significantly higher than the other ten in 13 protein-coding mitochondrial genes of. The 13 protein-coding gene splicing sequences Bayesian posterior rates were significantly higher than completed sequence, and were more suitable for Bayesian uncorrected relaxed lognormal clock analysis. Thirteen protein coding gene splicing sequence＇s Bayesian uncorrected relaxed lognormal clock work out snappers＇ two main branches broke out in 61.5 Ma before, which was the junction point of Danian and Selandian in Paleocene