以聚丁酸丁二醇酯为碳源去除含盐水体硝酸盐的研究

Denitrification of saline water with poly butylene succinate as carbon source and biofilm carriers

以可生物降解聚合物为碳源和生物膜载体可以解决异养反硝化有机碳源的添加不足或过量的问题。在序批式反应条件下,以聚丁酸丁二醇酯(PBS)为碳源和生物膜载体,对含盐水体异养反硝化过程中的细菌群落特征进行了研究。结果表明,试验条件下硝酸盐可以得到很好的去除,虽然有亚硝酸盐的明显积累,但最终被降低。硝酸盐的存在会降低含PBS水体中溶解性有机物的含量。应用变性梯度凝胶电泳和16SrDNA的方法鉴别到的细菌包括:Pseudomonas stutzeri,Pseudomonas sp.,Alteromonas sp.,Marinobacter salsuginis,Thalassospira xianheensis,Itellibacter vladivostokensis,Euplotopsis encysticus,Alcanivorax venusten-sis,Halomonas sp.,Agrobacterium tumefaciens,Pannonibacter phragmitetus,Vitellibacter vladivostokensis。试验结果表明,反硝化条件下PBS具有较好的可生物降解性和明显的NO3--N去除能力,是比较理想的低C/N含盐水体异养反硝化碳源。

The problem of improper addition of organic carbon source in heterotrophic denitrification process could be well solved by using biodegradable polymer as both solid carbon source and biofilm carriers. Batch experiments were carried out to use poly butylene succinate （PBS） as solid carbon source and biofilm carriers to remove nitrate-nitrogen from saline wastewater. The bacterial community was identified using denaturing gradient gel electrophoresis （DGGE） and 16S rDNA sequencing. The results showed that large amount of nitrate was removed under the experiment condition, although the obvious nitrite accumulation was found during denitrification process, but they eventually be removed. The existence of nitrate could reduce the content of dissolving organic matter in PBS containing wastewater. The bacteria found in bioreactor were assigned to Pseudomonas stutzri, Pseudomonas sp. , Alteromonas sp. ,Marinobacter salsuginis , Thalassospira xianheensis , Itellibacter vladivostokensis , Euplotopsis encysticus , Alcanivorax venustensis , Halomonas sp. , Agrobacterium tumef aciens , Pannonibacter phragmitetus , and Vitellibacter vladivostokensis. The experimental results showed PBS was available both for microbial attachment and for carbon source to treat the saline wastewater with low C/N ratio.