表面活性剂协同微波提取胡柚皮总黄酮的工艺研究

Study on Microwave-surfactant Extraction of Total Flavonoid from Pomelo Peel

目的优选表面活性剂增效微波提取胡柚皮中总黄酮的最佳工艺。方法对7种表面活性剂强化提取胡柚总黄酮的效果进行了比较,筛选出对该提取过程具有明显增溶效果的表面活性剂。以总黄酮含量为指标,采用正交试验确定最佳提取工艺。结果胡柚皮中的总黄酮的最优提取条件以质量百分数2%的月桂醇磺基琥珀酸单酯二钠盐(AK-201)为提取剂,微波频率为160 W,提取2次,每次1 min,提取2次,料液比为1∶40,提取率为4.35%。该工艺比仅用微波法提取胡柚皮中黄酮的提取率增加了6.36%。结论该提取工艺简单,方法快速、简便,能降低成本,同时提高提取率,可用于胡柚皮中总黄酮的提取。

OBJECTIVE To optimize the extraction technology of total flavonoids from pomelo peel by combining surfactant with microwave technology. METHODS As an indicator of total flavonoids, orthogonal test was used to study the extraction conditions to extract flavonoids from pomelo peel. RESULTS Optimum condition for extraction of pomelo peel was as follows： mass percentage of 2% lauryl sulfosuccinate disodium（AK-201） for the extraction solvent, microwave power 160 W, extracted twice for 1 min each time and the solid to liquid ratio was 1∶40. Using this technology, the extraction yield increased by 6.36% as compared with that using microwave technology alone. CONCLUSION This extraction process is steady, feasible and reproducible which can be used for extraction of total flavonoids from pomelo peel.