细胞膜红色荧光探针PLGA纳米粒的制备及表征

Preparation and Characterization of DiI Loaded PLGA Nanoparticles

目的以聚乳酸-羟基乙酸共聚物(PLGA)和单甲氧基聚乙二醇聚乳酸-羟基乙酸共聚物(MePEG-PLGA)为材料,制备包载细胞膜红色荧光探针(DiI)的纳米粒,为后续的细胞实验奠定基础。方法采用自乳化溶剂扩散法制备纳米粒;超滤法分离纯化纳米粒,紫外可见分光光度法测定DiI的含量,并计算包封率。结果 DiI用量为0.5 mg,PLGA或MePEG-PLGA投入量为50 mg,PVA浓度为0.5%,可制得粒径较合适的纳米粒。DiI-PLGA-NP平均粒径为(280.7±3.6)nm,Zeta电位为(2.98±0.47)mV,包封率可达88.0%;DiI-MePEG-PLGA-NP平均粒径为(157.2±3.2)nm,Zeta电位为(-4.90±0.54)mV,包封率可达87.1%。结论 DiI作为脂溶性良好的示踪剂,用其制得的荧光素纳米粒可为后续的体外实验奠定良好的基础。

OBJECTIVE To prepare DiI loaded nanoparticles using PLGA and MePEG-PLGA as carriers. METHODS Spontaneous emulsion solvent diffusion method（SESD） was adopt to prepare nanoparticles. Ultrafiltration-UV-visible spectrophotometry was used for the determination of DiI-NP＇ encapsulation efficiency. RESULTS When Dil＇s dosage was 0.5mg, PLGA＇s or MePEG-PLGA＇s dosage was 50 mg, PVA＇s content was 0.5%, the suitable nanoparticles were obtained. DiI-PLGA-NP＇ average diameter was （280.7士3.6）nm, DiI-PLGA-NP＇ Zeta potential was （-2.98士0.47）mV, DiI-PLGA-NP＇ encapsulation efficiency was 88.0%; DiI-MePEG-PLGA-NP＇ average diameter was （157.2士3.2）nm, DiI-MePEG-PLGA-NP＇ Zeta potential was （-4.90士0.54）mV, DiI-MePEG-PLGA-NP＇ encapsulation efficiency was 87.1%. CONCLUSION As a liposoluble long-term tracer, DiI-NP can lay the foundation for subsequent in vitro tests.