高频振荡通气结合低浓度一氧化氮吸入对急性缺氧大鼠肺动脉高压的作用

Effects of high frequency oscillation combined with nitric oxide inhalation on pulmonary hypertension caused by acute hypoxia in rats

目的 :观察高频振荡通气 (HFO)联合低浓度一氧化氮 (NO)吸入对急性缺氧大鼠肺动脉高压的治疗作用。方法 :在 HFO下 ,经气管插管给大鼠 12 %氧 30分钟制成急性缺氧肺动脉高压模型 ,行肺泡再充气 ,吸入低浓度 NO(10、5、2 mm ol/ L )对模型动物进行治疗 ,测定血流动力学参数和血气值。结果 :2～ 10 m mol/ L NO吸入均可逆转急性缺氧大鼠肺动脉压 (PAP) ,收缩压 (s PAP)从 4.44～ 4.5 3 k Pa(1k Pa=7.5 m m Hg)降至 3.38～ 3.48k Pa;平均压 (m PAP)从 2 .5 8～ 2 .87k Pa降至 2 .0 0～ 2 .18k Pa;舒张压 (d PAP)从 2 .2 0～ 2 .70 k Pa降至1.6 7～ 1.82 k Pa。NO吸入前后体循环压和心率的变化无显著性差异 (P均 >0 .0 5 )。Pa O2 、Pa CO2 、p H和肺内动静脉分流 (Qs/ Qt)均无显著性差异 (P均 >0 .0 5 )。结论 :HFO联合低浓度 NO(2 mmol/ L)吸入对大鼠急性缺氧性肺动脉高压有明显疗效 ;2～ 10 mm ol/ L NO吸入对急性缺氧大鼠肺动脉高压的降压效应与

Objective:To investigate the therapeutic effects of inhaled nitric oxide(NO) combined with high frequency oscillation(HFO) on pulmonary hypertension caused by acute hypoxia in rats.Methods:An animal model of pulmonary hypertension produced by acute hypoxia in rat was established by inspiration of 12% O 2 through tracheostomy tube for 30 minutes in HFO mode.The animals were then treated with alveolar recruitment strategy followed by the inhalation of low concentration of NO (10,5,2 mmol/L).Haemodynamic monitoring and blood gas analyses were performed accordingly.Results:Compared with hypoxia only,the pulmonary arterial pressure (PAP) of rats in NO inhalation groups were significantly decreased.The levels of systolic PAP(sPAP),mean PAP(mPAP),diastolic PAP(dPAP) were decreased from 4 444 53 kPa(1 kPa= 7 5 mmHg ) to 3 383 48 kPa,from 2 582 87 kPa to 2 002 18 kPa,from 2 202 70 kPa to 1 67 1 82 kPa ,respectively.No obvious changes were observed in mean systemic arterial pressure(MSAP),heart rate,pH,PaO 2,intrapulmonary shunt ratio(s/t),and PaCO 2 (all P>0 05) between hypoxia only and treatment groups.Conclusions:HFO+iNO (2 mmol/L) as a method of selective pulmonary vasodilation can be helpful for the treatment of pulmonary hypertension caused by acute hypoxia.The efficacy of NO on the pulmonary hypertension caused by the hypoxia is doseindependent (210 mmol/L).