ELISA法测定线粒体天门冬氨酸氨基转移酶及临床应用

ELISA for determination of Mitochondial Aspartate Aminotransferase and its Clinical Application

目的:建立ELISA法测定线粒体天门冬氨酸氨基转移酶方法及临床应用。方法:从心肌中纯化线粒体天门冬氨酸氨基转移酶,制备单克隆抗体,以双抗体夹心法规定酶蛋白。结果:方法的线性为0～150μg/L。批内变异为2.7％,批间变异为3.9％。回收率为99.3±3.4％。急性心肌梗死、急性肝炎、乙醇性肝炎线粒体天门冬氨酸氨基转移酶明显升高。结论:线粒体天门冬氨酸氨基转移酶为细胞坏死的指示酶,对急性心肌梗死、急性肝坏死的诊断及预后判定有重要价值。

Objective: To establish a enzyme - linked immunosorbent assay (ELISA) for measuring mitochondial aspartate aminotransferase (m - AST) and its clinical application. Methods: m - AST was purified from heart muscle and use to prepare monoclonal nmtibody. The protein enzyme concentration of m - AST in serum was measured by a simple 'sandwich' technique. Results: The lineary of method was 0-150 μg/L. The mean coefficient of variation were 2.7% within - assay and 3.9% between - assay. The analytical recovery was 99. 3 ± 3.4%. The m - AST mass concentration was markedly increased associated with acute myocardial infarction (AMI), acute hepatitis, alcoholic hepatitis. Conclusion: m - AST was a sensitive biochemical marker for cytonecrosisl. It was useful for diagnosis and prognosis of acute myocardial infarction and acute hepatic necrosis.