再生水处理过程微生物种群分析

Analysis of microbial populations in the process of recycled water treatment

以某再生水厂处理水为研究对象,对夏季(7月)水样进行了微生物多样性的分子生物学研究,结合变性梯度凝胶电泳(DGGE)分析了总进水、混凝沉淀出水、微滤出水、臭氧出水、反渗透出水和清水池出水中的微生物种群。应用PCR方法获得6个特异片段,并对其进行测序检索,分析了其可能代表的菌株,通过此方法获得了传统培养方法无法得到的菌株。研究发现,再生水处理过程中存在的菌种有鞘氨醇单胞菌、黄杆菌属和假单胞菌属,前两者可降解有机污染物,后者具有脱氮作用;同时上述细菌也具有一定的致病性和腐蚀性,若出厂水中含有上述细菌,则因其在再生水管网中的繁殖,有可能导致供水水质恶化,加速管网腐蚀。因此,水厂出水应加强消毒,控制微生物数量,保证再生水输配管网的供水安全。

The water treated in a recycled water plant has been taken as a target of research, and the research on mi- crobial population diversity of molecular biology has been carried out for the water samples in summer （July）. Com- bined with DGGE, microbial population diversity of the total influent, coagulation sedimentation effluent and micro- filtration effluent, ozone effluent, RO effluent and the water in clean water tank are analyzed. Six kinds of specific fragments are obtained by PCR, and order-checking and searching work needs to be done. The strain that might pos- sibly represent is analyzed. By this method, the strains which could not be obtained by traditional methods, has been obtained. It is found in this research that the strains existed in the process of recycled water treatment are Sphin- gomonas, Chryseobacterium and Pseudomonas. The first two kinds of them are capable of degrading organic pollu- tants;and the third one has denitrifying capability. At the same time,the above bacteria are pathogenic and corro- sive. If the effluent water from the plant contains the above bacteria, the water quality of the supplied water might be worsened, and the corrosion of pipe network gets faster, due to the propagation of the bacteria in the pipe network of recycled water. Therefore, the quantity of microbia should be controlled by strengthening the disinfection in water plants, so as to insure the security of recycled water supply.