早食李基因组DNA提取方法的改进

Improvement of Technique for Extraction of Total DNA from "Zaoshi" Plum

以改进的CTAB法对三华李品种群中的早食李叶片基因组DNA的提取进行了试验。结果表明,改进的CTAB法适于高质量三华李品种群基因组DNA的提取,提取的早食李基因组DNA完整性较好,无降解,OD260/OD280的比值在1.9～2.0之间,多糖类杂质去除较为干净。经SRAP检验,条带清晰,多态性良好,说明改良CTAB法提取的基因组DNA质量较好,适于三华李高质量基因组DNA的提取。不同时期的不同成熟度的叶片都可以提取到基因组DNA,但以各期的嫩叶(梢)产量最高,效率最好。

The DNA extraction experiment on ＂Zaoshi＂ plum was carried out by improved CTAB DNA extraction method. The results indicated that the improved CTAB DNA extraction method was suitable for extraction of high quality DNA from the ＂Sanhua＂ plum cultivar group. The extrac- ted ＂Zaoshi＂ plum DNA was in good integrality and without degradation. The OD260/OD280 radio was between 1. 9 - 2. 0, and the polysaccharide was dislodged clearly. The SRAP test produced clear polymorphic patterns, which indicated that the improved CTAB DNA extraction method was suitable for high quality DNA extraction from ＂Sanhua＂ plum cultivar group. The leaves at different development stage were also suitable for DNA extraction, but the young leaves （shoots）were the most suitable.