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侵染垂花悬铃花的木尔坦棉花曲叶病毒分子特征研究 被引量:20

Molecular characterization of the Cotton leaf curl Multan virus infecting Malvaiscus arboreus
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摘要 垂花悬铃花曲叶病是近期在广东发现的一种新病害,病株表现为叶片向上卷曲,叶脉肿大,叶脉变深绿色等症状。PCR检测结果显示,该病样中均存在菜豆金色花叶病毒属病毒。基因克隆及序列分析结果表明,该病毒分离物(GD11)DNA-A全长为2 737 nt,具有菜豆金色花叶病毒属病毒基因组典型特征,为闭合环状单链DNA,编码6个ORFs;该序列与木尔坦棉花曲叶病毒(CLCuMV)各分离物序列的相似性均大于89.0%,其中与G6、Okra06及GX1等分离物序列的相似性大于99.0%。该病毒分离物也伴有卫星DNAβ分子,其全长为1 348 nt,与CLCuMV各分离物的DNAβ序列相似性大于85.0%,其中与G6、Okra06及GX1等DNAβ的序列相似性均大于99.0%。因此,侵染广东垂花悬铃花的病毒分离物属于CLCuMV,且与入侵我国的朱槿分离物G6、黄秋葵分离物Okra06及棉花分离物GX1亲缘关系很近。本文首次报道了CLCuMV及其卫星β复合侵染垂花悬铃花。 Malvaiscus arboreus leaf curl disease in Guangdong is a new disease. The diseased plants exhibits leaf curling upwards, vein swelling and vein dark green. The results of PCR detection indicated that the diseased plants were infected by a begomovirus. Virus isolate GDll was obtained from M. arboreus leaf curl disease in Guangzhou, Guangdong Province. The complete nucleotide sequence of DNA-A was determined to be 2 737 nucleotides, encoding six potential ORFs. It was typical characteristics of Begomovirus. Comparisons showed that the DNA-A of GD11 had more than 89.0% sequence identity with all other isolates of Cotton leaf curl Multan virus (CLCuMV), and more than 99.0% sequence identity with isolates G6, Okra06 and GX1. The virus was also associated with satellite DNA 13 molecular, which contained 1 348 nucleotides. Pairwise comparison indicated that the DNAβ of GDll had more than 85.0% sequence identity with CLCuMV DNA β, and more than 99.0% sequence identify with isolates G6, Okra06 and GX1. These suggested that isolate GDll infecting M. arboreus in Guangdong belonged to CLCuMV. Moreover it was most closely related to the isolate G6 infecting Hibiscus rosa-sinensis, the isolate Okra06 infecting Hibiscus esculentus in Guangdong and the isolate GX1 infecting Gossypium hirsutum in Guangxi. The paper is the first report on CLCuMV and its DNA β complex infecting M. arboreus.
出处 《植物病理学报》 CAS CSCD 北大核心 2013年第2期120-127,共8页 Acta Phytopathologica Sinica
基金 国家自然科学基金(31171817) 国家国际科技合作项目(2011DFB30040) 广东省自然科学基金(9151065005000010 S2011040003309) 广东省科技计划项目(2011B050400003)
关键词 垂花悬铃花曲叶病 木尔坦棉花曲叶病毒 DNA—A 卫星DNAΒ Malvaiscus arboreus leaf curl disease Cotton leaf curl Multan virus (CLCuMV) DNA-A satellite DNA β
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