重组L-天冬酰胺酶的鉴定

Appraisal of Recombinant L-asparaginase

目的 :对重组L 天冬酰胺酶进行鉴定。方法 :用基因测序、氨基酸组成分析、N 末端氨基酸测序、胰肽图谱、IEF、分子量、紫外扫描等各种实验手段对重组L 天冬酰胺酶及其天然品进行了全面比较。结果 :重组产品与天然品具有同质性。结论 :重组L 天冬酰胺酶具有较好的应用前景。

Purpose:To give an appraisal of recombinant L-asparaginase.Methods:Various analytical techniques including gene sequencing,amino acid composition,analysis of N terminal amino acid analysis,peptide mapping,IEF,molecular weight and UV scanning were used.Results:The recombinant L asparaginase had the same quality as that of native products.Conclusion:The application of recombinant L-asparaginase was promising.