E1A基因对人肺腺癌细胞增殖和转移的影响

Effect of adenovirus type 5 E1A gene on proliferation and metastasis of human lung adenocarcinoma cells

目的 探讨腺病毒 5型早期区 1A(Ad5E1A)基因对人肺腺癌细胞增殖和转移的影响 ,为人肺腺癌E1A基因治疗的可行性提供实验依据。方法 用本室构建的真核表达重组质粒pcDNA3 E1A ,通过脂质体介导将E1A基因转入人肺腺癌细胞系 (Anip 973) ,经G418筛选获得稳定表达E1A基因的转染细胞 (Anip 973 E1A)。通过体内、外实验 (生长速度、倍增时间、软琼脂集落形成、裸鼠致瘤性及转移能力等 ) ,观察E1A基因对人肺腺癌细胞增殖和转移的影响。结果 体外实验显示 ,Anip 973 E1A细胞生长速度减慢 ,倍增时间延长 ;软琼脂集落形成能力显著降低 ,亲本Anip 973、Anip 973 vect和Anip 973 E1A细胞集落形成率分别为 19.7%、17.2 %和 2 .3% ,集落形成抑制率为 86 .6 %。体内实验显示 ,Anip 973 E1A细胞组出瘤时间晚 ,肿瘤体积小 ,有 2只裸小鼠至实验结束未触及肿瘤 (2 /6 ) ,抑瘤率为 6 0 .1%。第 2 1天计数肺转移灶 ,亲本Anip 973、Anip 973 vect和Anip 973 E1A细胞组肺转移灶数目分别为 16 .1± 3.2、15 .4± 3.8和 7.3± 2 .3个 ,转移抑制率为 5 2 .6 %。结论 E1A基因能显著抑制人肺腺癌细胞的恶性表型 ,降低裸鼠致瘤性 ,减少肺转移灶形成 ,为人肺腺癌E1A基因治疗提供了实验依据。

Objective To investigate the effects of E1A gene on proliferation and metastasis of human lung adenocarcinoma cells.Methods A mammalian E1A expressing recombinant constructed in our laboratory, named pcDNA3 E1A, was introduced into human lung adenocarcinoma cell line(Anip 973)by lipofectamine. The cells resistant to G418 were selected. The characteristics of the E1A expressing Anip 973(Anip 973 E1A) cells were studied in vitro and in vivo, including cell growth rate and doubling time, colony forming efficiency on soft agarose, tumorigenicity and metastasis.Results The growth rate of E1A Anip 973 cells was diminished and their colony forming efficiency was inhibited significantly. The tumorigenicity in nude mice of the E1A expressing Anip 973 was markedly suppressed.Conclusion E1A suppresses the malignant phenotype of human lung adenocarcinoma cell line. This study provides experimental data for gene therapy of lung cancer with E1A.