癌症患者外周血淋巴细胞银染核仁形成区变化规律研究

Research on the regular pattern of Ag NOR changes in patients with cancer

目的 用核仁银染强度探讨肿瘤患者外周血Th淋巴细胞rDNA转录活性与机体免疫状况的关系。方法 应用计算机图像分析系统 ,对癌症、炎症、非癌非炎性疾病患者及正常人外周血Th(CD4+ )淋巴细胞中的核仁银染强度进行比较。结果 与炎症、非癌非炎症疾病患者及正常人相比 ,癌症患者Th淋巴细胞核仁银染强度显著下降。结论 癌症患者淋巴细胞rDNA转录活性可以灵敏地反映体内癌变进展程度与机体免疫状况的相互关系 ,有可能为临床诊断癌症提供一个简便可行的辅助方法。

Objective To correlate the relationship between rDNA transcriptional activity of CD4 + helper T cells and immunity in patients with cancer through silver stainability of nucleolus organizing regions (Ag NORs).Methods The Th(CD4 +) rDNA transcriptional activity was compared among patients with cancer, infectious diseases, non cancerous non infectious diseases through image analysis.Results Th(CD4 +) rDNA transcriptional activity was decreased extremely in patients with cancer, but increased in patients with inflammation compared with normal controls.Conclusion The analysis of Th(CD4 +) rDNA transcriptional activity may serve as a new tumor marker for tumor diagnosis and differential diagnosis. Therefore, it may provide an innovative and reliable method for clinical cancer diagnosis.