寒凝血瘀型原发性痛经与MHC基因多态性的关联研究

MHC -DRB1 ＊ gene Polymorphism in Patients with Primary Dysmenorrhea

目的:探讨北方地区汉族人寒凝血瘀型原发性痛经(PD)与人类主要组织相容性复合MHC基因多态性的关系。方法:应用顺序特异性引物和聚合酶链反应(PCR-SSP)技术,对64例原发性痛经患者及85例无血缘关系的健康人的MHC各等位基因及亚基因进行了检测分析,并将该方法与其它检测MHC等位基因的方法进行对比。结果:结果表明,MHC-DRB1*09(RR=4.202,P<0.05)基因与PD正相关,显著高于对照组,两组之间比较差异有显著性意义,而其它MHC-DRB1*各等位基因未见异常,均无统计学差异。结论:本项研究结果提示,MHC-DRB1*09基因可能是我国北方汉族人PD致病的易感基因,为揭示PD的发病机制中免疫遗传学作用提供了重要信息和依据。

Objective：In order to study the correlativity between MHC - DRB1 ＊ gene polymorphism and Primary Dysmenorrhea （PD）. Methods： MHC - DRB1＊ gene Polymorphism in 64 patients withPD and 85 normal control subjects was analyzed by using the polymerease chain reaction／sequence specific primer （PCR／SSP） technique. 85 healthy people that not related by blood as controlled group, they were examined by the same method. Results： MHC- DRB1 ＊09（ RR = 4. 202 ,P 〈 0. 05 ）. The frequency of MHC - DRB1 ＊ 09 allele was higher than controlled group remarkably, the difference between two groups was significant in statistics, the others allele of MHC -DRB1 ＊ were no abnormality seen, there was no difference in statistics. Conclusions：These findings further Verify that immunogenetics play a predominant Patho- genetics role in a Subset of PD patients. Individuals carrying MHC - DRB1 ＊ 09 alleles may be susceptibility gene to PD. This method is more specific, sensitive, simple, rapid and accurate, and could be applied to the routine clinical detective.