前列腺增生症肾虚血瘀证大鼠动物模型的建立与评价

Establishment and evaluation of benign prostatic hyperplasia in rats with deficiency of kidney and blood stasis animal model

目的:建立前列腺增生症(BPH)肾虚血瘀证大鼠动物模型并进行评价。方法:采用去势手术、激素注射加冰浴方法制作BPH肾虚血瘀证大鼠模型,考察前列腺湿重、前列腺指数和血清前列腺特异性酸性磷酸酶(PAP)含量,观察模型大鼠病理组织形态学。结果:模型组大鼠全血黏度、血浆黏度、红细胞聚集指数和卡松黏度均明显高于空白组和假手术组(P<0.05);模型组血清PAP的含量升高(P<0.05),并且模型组大鼠前列腺组织病理组织学观察可见小血管扩张、充血,符合瘀血的病理表现。结论:该模型制作方法简单,评价指标客观,为BPH肾虚血瘀证以及相关中药治疗机制的研究提供了良好的实验基础和理论依据。

Objectives：To establish and evaluate benign prostate hyperplasia rat animal model with blood stasis due to kidney deficiency. Methods：Using the castration surgery, hormone injections and ice bath method manufacturing BPH with kidney deficiency and blood stasis rat model; this paper investigated the prostate wet weight, prostate index and the content of serum prostate specific acid phosphatase （PAP）, and observed model rats pathological tissue morphology. Results： The whole blood viscosity, plasma viscosity, erythrocyte gathered index and Casson viscosity of rats in model group were significantly higher than that in blank group and sham-operated group, and the difference was statistically significant （P 〈 0. 05 ）. The content of serum PAP in model group was increased （P 〈 0. 05 ） , and prostate tissue had been observed visible small blood vessels and congestion, according with blood stasis, thus it is demonstrated that the blood stasis rats model had been done. Conclusions ：The rat model for BPH with kidney deficiency and blood stasis was copied successfully. Production method is simple and the evaluation index is objective, which provides the good experimental foundation and theoretical basis for the study of BPH with kidney deficiency and blood stasis syndrome and the theraputic mechanism of related Traditional Chinese Medicine.