中药复方962对老龄大鼠皮质胆碱乙酰转移酶和乙酰胆碱酯酶活性的影响

Effects of Traditional Chinese Compound 962 on Choline Acetyltransferase and Acetylcholinesterase in Cerebral Cortex of Aged Rats

为研究中药复方 962胶囊对老龄大鼠皮质胆碱乙酰转移酶 (ChAT)和乙酰胆碱酯酶 (AchE)活性的影响 ,将2 2月龄和 2 4月龄Wistar大鼠分为青年对照组、老年模型组、阳性对照脑复康组 ( 0 .56g/kg)、962中剂量组 ( 0 .9g/kg)和 962大剂量组 ( 1 .8g/kg) 5组 ,分别灌胃给药 1～ 2个月后处死大鼠。用Fonnun法和羟胺比色法分别测定 5组大鼠皮质的ChAT和AchE的活性。结果 :中药复方 962大剂量 ( 1 .8g/kg)对老龄大鼠皮质ChAT活性有提高作用 (P <0 .0 5) ,但对AchE无显著影响。提示 :中药复方

In order to determine the effect of the compound 962 capsule on the choline acetyltransferase and acetylcholinesterase in cerebral cortex of aged rats, and to probe the anti agedness mechanism from the view of choline energy nerve. Male and female aged rats( 22 month old and 24 month old) were classified into five groupsyoung control group, old model group, piracetem group (0.56 g/kg), 962 middle dose group (0.9 g/kg) and 962 high dose group (1.8 g/kg). All test drug was administrated for 1～2 months by ig. The choline acetyltransferase and acetylcholinesterase activity were measured by Fonnun and hydroxylamine colorimetry method. Results: Compound 962 improved the activity of choline acetyltransferase ( P < 0.05 ), but it had little effect on the acetylcholinesterase in cerebral cortex of aged rats. This indicated that by affecting the central nervous system in aged rats, compound 962 capsule could improve the aging process.