摘要
目的研究剑叶三宝木(Trigonostemon xyphophylloides)枝条乙醇提取物的化学成分。方法采用硅胶柱色谱、Sepha-dex LH-20凝胶柱色谱、半制备高效液相色谱进行分离纯化,根据理化常数和波谱数据鉴定化合物的结构。结果从剑叶三宝木中分离得到8个化合物,分别鉴定为:Δ8,22-麦角甾烯-3β-醇-11-酮(1)、mangdesisterol(2)、6-Deoxy-jacareubin(3)、ergosterol peroxide(4)、丁香脂素(5)、表丁香脂素(6)、β-谷甾醇(7)和胡萝卜苷(8)。结论化合物1为一新天然产物,化合物2、3、4、6和8为首次从该属植物中分离得到,化合物5为首次从该植物中分离得到。用滤纸片琼脂扩散法测定了化合物1~6的抗菌活性,结果显示化合物1,2和6对金黄色葡萄球菌有抑制作用。
Objective To study the chemical constituents of the ethanol extract from the twigs of Trigonostemon xyphophylloides.Methods silica gel,sephadex LH-20 colunm chromatography,and Semi-preparative HPLC were employed for the isolation and purification.The structures of compounds were elucidated by the physico-chemical properties and spectral analyses.Results 8 compounds were identified as follows:Δ8,22-ergostadien-3β-ol-11-one(1),mangdesisterol(2)6-Deoxyjacareubin(3),ergosterol peroxide(4),syringaresinol(5),episyringaresinol(6),β-daucosterol(7),and daucosterol(8).Conclusion Compound 1 was a new natural product.Compounds 2,3,4,6,and 8 were isolated from the genus Trigonostemon for the first time.Compound 5 was isolated from this plant for the first time.Compounds 1,2,and 6 showed inhibitory effect on Staphylococcus aureus by paper disk diffusion method.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2013年第3期591-593,共3页
Lishizhen Medicine and Materia Medica Research
基金
中国热带农业科学院中央级公益性科研院所基本科研业务费(No.CATAS-ITBB 110403)
关键词
剑叶三宝木
化学成分
抗菌活性
Trigonostemon xyphophylloides
Chemical constituents
Antibacterial activity