解毒活血法对UUO大鼠MCP-1的影响

Effects of Jiedu Huoxue Therapy on the MCP-1 in Rats with Unilateral Ureteral Obstruction

目的采用单侧输尿管梗阻的方法建立肾小管间质纤维化模型,通过观察解毒活血法对大鼠肾组织单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)的影响,探讨其对UUO大鼠的保护作用及可能机制。方法 SD大鼠随机分为假手术组,模型组,缬沙坦组,解毒活血低剂量组,解毒活血高剂量组,每组10只。除假手术组,其余各组大鼠结扎左侧输尿管复制UUO模型。所有大鼠灌胃给药,缬沙坦组给予26 mg/(kg.d);解毒活血低剂量组予解毒活血中药12 g生药/(kg.d);解毒活血高剂量组予24 g生药/(kg.d),假手术组和模型组予等容量生理盐水,共14天。左肾组织行HE、Masson染色,观察病理形态学改变,免疫组化检测肾组织中MCP-1的蛋白表达。结果模型组大鼠MCP-1呈强阳性表达,各治疗组表达较模型组明显减弱。结论解毒活血方药可降低肾组织中MCP-1的高表达,通过抑制炎症反应来减缓肾小管间质纤维化的进程。

Objective Animal model with TIF made by unilateral ureteral obstruction（UUO）,the study is to observe the effects of Jiedu Huoxue therapy on the expression of monocyte chemoattractant protein-1（MCP-1） in UUO rats,and to explore the protective effect and possible mechanism of Jiedu Huoxue therapy in UUO rats.Methods Fifty Sprague-Dawley rats were randomized equally into 5 groups：Control group,UUO group,Valsartan group,low-dose of Jiedu Huoxue decoction group and high-dose of Jiedu Huoxue decoction group.Except the Control group,rats in other four groups were made into UUO model by ligaturing the left ureter.All rats were treated with drugs by oral infusion once a day.Rats in Valsartan group,Jiedu Huoxue decoction low-dose group and high-dose group were treated respectively with Valsartan 26mg/kg·d,Jiedu Huoxue decoction 12g/kg·d and 24g/kg·d for fourteen days.The protein expressions of MCP-1 in rats＇ kidney were detected by immunohistochemical.Results The expression of MCP-1 were increased significantly in the model group.After treatment,the expression of MCP-1 were significantly down-regulated in the treatment groups.Conclusion Jiedu Huoxue decoction is able to reduce the high expressions of MCP-1,and to retard the progress of renal tubular interstitial fibrosis by inhibiting inflammatory reaction.