大鼠慢性心衰心阳虚型模型的建立

Establishment of Chronic Heart Failure Rat Model with Heart Yang Deficiency

目的:在现有模型的制作方式上加以改良,建立更稳定、更经济的慢性心衰心阳虚型动物模型。方法:采用以阿霉素诱导为主,配合每天低温刺激的复合模型来模拟阳虚表现,使用心脏彩超同步检测正常组、模型改良组、模型1组、模型2组、冷冻组的LVEDD、LVESD、LVEF、FS心功能变化。结果:在造模过程中,改良组与模型1、2组大鼠均逐渐出现一系列阳虚症状,改良组较模型1、2组明显。造模结束后,分别比较各组体重、生存率、LVEDD、LVESD、LVEF、FS的变化。改良组与模型2组各指标变化符合慢性心力衰竭心阳虚证动物模型证候标准,改良组与模型2组各心功能指标间差异无统计学意义(P>0.05),与模型1组间差异具有统计学意义(P<0.05)。结论:模型改良组造模用时短,成功率高,可作为各种慢性心衰心阳虚型大鼠实验的造模参考方法。

Objective： To improve the method of the existing model making in order to establish an animal model of de- ficiency of heart-YANG in CI-IF with more stable and more economical.Methods： Adriamycin induction used mainly with low temperature stimulation daily as a complex model simulates the symptoms of deficiency of yang. UCG is used synchronously to measure the LVEDD, LVESD, LVEF and FS of the heart function in the normal, model-improved, model-l, model-2 and frozen groups. Results： In the process of model-building, the rats gradually present a series of symptoms of deficiency of yang in model-improved, model-1 and model-2 groups and those symptorps are more obvious in model-improved group. When the model-building is finished, the data of body weight,survival rate, LVEDD, LVESD, LVEF and FS of every group is for com- parison. The change of index are correspond with syndrome standard of animal model syndrome of deficiency of heart-YANG in CHF in the＂ model-improved and model-2 group. There is no statistically significant variance in the data between the mod- el-improved and model-2 group（P〉0.05）, but there was y significant difference between the model-improved and model-1 （P〈 0.05）. Conclusions： Model-Improved group is built with less time and higher success rate. It can be a referential method in building a range of rat experiment of deficiency of heart-YANG in CHF.