不同提取方法考察盘龙参药材山奈酚含量测定

Research on Kaempferol Content from Spiranthes australis by Different Extract Methods

目的:以山萘酚含量为指标,探讨盘龙参的合适提取方法。方法:盘龙参全草用95%乙醇溶液,经索氏、超声和回流3种提取方法,分别对提取液进行HPLC法测定山萘酚的含量。结果:索氏、超声和回流3种不同提取方法,所得到的山萘酚的含量(mg/g)分别为2.528、0.812、0.968。结论:索氏取方法为从盘龙参药材中提取黄酮的最佳方法。

Objective： To study Spiranthes australis suitable extraction method with kaempferol content as the index,. Methods： Panlong ginseng herbs with 95% ethanol solution, by Soxhlet, ultrasonic and reflux three extraction methods respec- tively, kaempferol content in extract were determined by HPLC chromatogrophy. Results： The kaempferol content （mg/g） were 2.528, 0.812, 0.968 in Soxhlet, ultrasonic and reflux respectively. Conclusion： Soxhlet extraction method for extracting flavone from Panlong ginseng herbs was optimal method.