白藜芦醇对不同乳腺癌细胞增殖的抑制作用

Inhibition of resveratrol on proliferation of different breast cancer cell lines

目的探讨白藜芦醇（Res）对雌激素受体（HE）表达不同的3种乳腺癌细胞DU4475、MDA—MB-231和MCF-7增殖、周期和凋亡的影响。方法药物处理组加入终浓度为6．25、12．5、25、50、100、200μmol／L的Res，分别设24、48、72h3个时期。采用噻唑蓝比色法（MTT）法检测Res对乳腺癌细胞增殖的影响，流式细胞术（FCM）检测细胞周期及凋亡，药物浓度为0、12．5、25、50μmol／L。应用统计软件SPSS17．0进行统计学处理。结果经Res作用后，3种乳腺癌细胞增殖均受到不同程度抑制。其中Res作用48h和72h，各浓度对细胞的抑制均有统计学意义（F=15．26，P〈0．05），对ER阴性DU4475和MDA-MB-231细胞株的抑制率均高于ER阳性的MCF-7细胞株。FCM检测结果显示，Res与乳腺癌细胞作用48h，Res浓度在12．5—50μmol／L时，3种乳腺癌细胞周期均被阻滞于S期，阻滞比例差异有统计学意义（F=34．81，P〈0．05），DU4475和MDA—MB-231阻滞于S期的比例高于MCF-7。100μmol／L时，Res对DU4475的促凋亡作用明显强于MCF-7细胞（t=16．082，P〈0．05）。结论Res可抑制以上3种乳腺癌细胞增殖，导致细胞周期改变，促进凋亡。Res对DU4475和MDA—MB-231细胞增殖抑制作用强于ER阳性MCF-7细胞。

Objective To investigate the effects of resveratrol （Res） on the proliferation, cell cycle and apoptosis of three breast cell lines DU4475, MDA-MB-231 and MCF-7 with different estrogen receptor （ER） expressions. Methods Res was added to the drug treatment group at 6.25, 12.5, 25, 50, 100 and 200 μmol/L, respectively. Three observation periods at 24, 48 and 72 hours were set up respectively. MTY assay was used to detect the effects of Res on proliferation of breast cells. Cell cycle and apoptosis were analyzed by flow cytometry （FCM）. The concentrations of drugs were 0, 12.5, 25, 50 μmol/L. The results were analysed by the statistical software SPSS17.0. Results After Res intervention, the proliferations of three cell lines were inhibited to different extent. After 48 and 72 hours of Res, inhibitions of Res with different concentration were significant different （ F = 15.26, P 〈 0.05）. Inhibition rates of DU4475 and MDA-MB-231 with ER-negative were higer than that of MCF-7 with ER-positive. FCM rcsuhs prompted that these three kinds of cells were blocked in S phase after 48 hours treatment of 12.5-50 μmol/L Res and the block percentages had significant difference （ F = 34.81, P 〈 0.05 ）. The percentages of S phase for DU4475 and MDA-MB-231 arresting were higher than that of MCF-7. For DU4475, the apoptotic and necrosis percentage were higher than that of MCF-7 at 100 μmol/L （ t = 16. 082, P 〈 0.05 ）. Conclusion Res can inhibit proliferation, induce cell cycle changing and apoptosis of DU4475, MDA-MB-231 and MCF-7 cells. The inhibitions of Res on DU4475 and MDA-MB-231 are better than that of MCF-7 with ER-positive.