金电极上二硫醇自组装单层的制备及其用于纳米结构生物传感界面的构建

Preparation of SAMs of dithiol on the gold electrode for construction of nano-structured biosensing interface

生物电化学连接生物技术和电化学科学，纳米技术打开了这个领域科学研究新境界。由于许多生物活性分子，例如细胞色素C的氧化还原中心通常位于生物分子内部，所以其氧化还原中心与电极之间的直接电子传递很难实现。本文首先通过在金电极上分别修饰己二硫醇和苯二硫醇的自组装单层SAMs，然后再将修饰了金纳米颗粒（AuNPs）的多壁碳纳米管（MwcNTs）固定于SAMs上，然后再修饰上细胞色素C，利用金纳米颗粒和碳纳米管良好的导电性和宏观隧道效应，实现了对细胞色素C的直接电化学。并将该修饰电极用于对H202的催化研究。

Bioelectrochemistry combines bioteehnology with electrochemical science, nanotechnology is opening new horizons for the in- vestigation of this fieldo Because many bioactive molecules, such as Cytohrome C and protein, their redox site is often hidden deep inside the biomolecule, it＇ s hard to achieving direct ET between redox site and electrode. In this paper, firstly, gold electrode modified by SAMs of 1,6-Hexanedithiol and 1,2-Benzenedithiol respectively. Then AuNPs/MWCNTs fixed on SAMs, and then modified with cytochrome C. Use of good conductivity of gold nanoparticles and macroscopic tunnel effect of carbon nanotubes, achieved direct electrochemistry of cytochrome C.and use the modified electrode for catalytic studies of H2O2.