苯海索对大鼠蛛网膜下腔出血后脑血管痉挛的预防作用

Prophylactic effects of trihexyphenidyl on cerebral vasospasm after subarachnoid hemorrhage in rats

目的 :为了探讨苯海索对蛛网膜下腔出血(SAH)后脑血管痉挛及自由基损伤的预防作用。方法 :采用大鼠蛛网膜下腔出血模型 ,放射性生物微球法测定SAH后脑皮质局部血流量 (rCBF) ,分别采用肾上腺素自氧化法和硫代巴比妥酸法检测脑皮质超氧化物歧化酶 (SOD)和脂质过氧化物 (LPO)的水平。结果 :SAH后 4 8h时脑皮质血流量降低 57.7% (P <0 .0 1) ,LPO含量显著升高 (P <0 .0 1) ,SOD活力明显降低 (P <0 .0 5) ;苯海索可显著增加SAH后脑皮质血流量 (P <0 .0 1)和减少LPO的生成 (P<0 .0 1)。结论 :苯海索可有效防止SAH后脑血管痉挛的发生 ,增加rCBF ,并减轻脑皮质的自由基损伤。

AIM: To study the prophylactic effects of trihexyphenidyl(THP) on cerebral vasospasm and damages of free radicals after subarachnoid hemorrhage(SAH). METHODS: SAH models of rat were employed, the regional cerebral blood flow (rCBF) in the cerebral cortex was determined by radioactive biomicrosphere assay; the activity of superoxide dismutase(SOD) and concentration of lipid peroxides (LPO) in the cerebral cortex were assayed by adrenaline autoxidaton and thiobarbituric acid procedure respectively. RESULTS: Compared with control group, rCBF in cerebral cortex of SAH group decreased by 57.7 % ( P <0.01) at 48 h after SAH, and there was an extensive increase in LPO concentrations ( P <0.01) and a remarkable decrease in SOD activities ( P <0.05). THP could effectively elevated the rCBF ( P <0.01) after SAH, and remarkably inhibited the LPO ( P <0.01) producing. CONCLUSION: THP could significantly prevent cerebral vasospasm, increase rCBF, and decrease damages of free radicals to cerebral cortex after SAH.