荧光原位杂交技术检测慢性B-CLL第12号染色体三体畸变

DETECTION OF TRISOMY 12 IN CHRONIC B LYMPHOCYTIC LEUKEMIA (B CLL) BY FLUORESCENCE IN SITU HYBRIDIZATION

目的 检测 2 0例慢性 B淋巴细胞白血病 (B-CL L )中第 12号染色体三体畸变。方法 运用荧光原位杂交技术 (FISH )。结果 6例 (30 % )患者有 12号染色体三体 (+12 ) ,其中 5例经染色体核型分析发现的 + 12均得到证实。在 1例 B- CL L 中 ,染色体分析未发现任何分裂相 ,而 FISH检测发现了 + 12克隆。在另 1例 B- CL L 中 ,FISH不仅证实了染色体核型检查发现的存在于扁桃体的 + 12 ,同时还发现了染色体分析未发现的在外周血中的 + 12克隆。结论 本实验结果表明

Objective To detect trisomy 12 in 20 patients with B CLL.Methods Using fluorescence in situ hybridization (FISH) technique with a centromeric probe for chromosome 12. Results Trisomy 12 was detected in 6 (30%) of 20 B CLL, and in 5/6 cases trisomy 12 revealed by chromosome karyotyping was confirmed by FISH study; in another case, chromosome analysis found no mitosis, while FISH identified a trisomy 12 clone still in another B CLL, cytogenetic study found +12 only in tonsil, while FISH not only proved +12 in tonsil but also in peripheral blood cells. Conclusion The study showed that FISH was more sensitive than traditional cytogenetics in detection of chromosome aberrations.