急性白血病LRP、mdr_1、MRP基因表达的预后意义

THE PROGNOSTIC SIGNIFICANCE OF LRP,MDR_1,MRP GENE EXPRESSION IN PATIENTS WITH ACUTE LEUKEMIA

目的 研究急性白血病 (AL)患者肺耐药蛋白(L RP)基因、多药耐药基因 mdr1 及多药耐药相关蛋白(MRP)基因表达的预后意义。方法 急性白血病 6 1例。正常对照 15例。采外周血或骨髓液 ,肝素抗凝 ,分离单个核细胞及分离总 RNA。逆转录酶合成 c DNA。设计扩增 L RP、mdr1 及 MRP基因的引物进行 PCR扩增。结果 正常对照及长期生存组三种耐药基因表达的阳性率均很低 ;初治组表达的阳性率较高 ;且初治组耐药基因表达阳性者 ,其完全缓解率 (CR)显著低于表达阴性者 ;复发难治组表达水平显著高于前三组 (P<0 .0 5 ) ;耐药基因的表达与 FAB分型有关。结论 AL患者 L RP、mdr1 和

Objective To study the prognostic significance of LRP, mdr 1,MRP gene expression in patients with acute leukemia.Methods Heparinized blood or bone marrow samples were obtained from 61 cases of acute leukemia and 15 normal controls. Monouclear cells were separated by the acid guanidium phenol chloroform method. The cDNA was synthesized using reverse transcriptase. The four pairs of primers chosen from the sequences of LRP, mdr 1,MRP and β 2 were used for the PCR. Results The positive percentrage of LRP. mdr 1 and MRP gene expression in relapsed refractory group were significantly higher than those in untreated, long term survival and normal control group ( P <0 05)。The 1st complete remisson (CR 1) rate of LRP mdr, MRP negative patients were obviously higher than positive ones in the untreated group. These multidrug resistance gene expressions were correlated with FAB classification. Conclusion High expression of LRP, mdr 1 and MRP gene is related to clinical multidrug resistance. Therefore, it may be regarded as an important factor of prognosis of patients with acute leukemia.