长期酒精暴露对秀丽隐杆线虫生殖能力的损伤作用研究

Study of the reproductive injury of Caenorhabditis elegans induced by alcohol with a long-term exposure

目的应用秀丽隐杆线虫研究酒精的生殖损伤作用及其对子代线虫运动能力的影响。方法将L4期秀丽隐杆线虫暴露在含有不同浓度酒精(1%、2%、3%、4%、5%)并涂有大肠埃希菌OP50的琼脂培养基(NGM)中,在体视显微镜和荧光显微镜下统计后代数目、子宫内受精卵数目、卵母细胞数目,计算排卵速率并观察子代线虫的运动行为。结果与对照组比较,长期暴露在含酒精浓度分别为2%、3%、4%、5%的NGM培养基中的线虫的后代数目、子宫内受精卵数目、卵母细胞数目均有显著降低(P<0.05);含3%、4%、5%酒精的NGM培养基中的线虫排卵时间和排卵速率均有显著降低(P<0.05);各实验组中孵化的子代线虫的头部摆动频率和身体扭动频率均有显著降低(P<0.05)。结论长期酒精暴露对秀丽隐杆线虫生殖能力有一定的损伤作用,而且可能对子代线虫的运动能力造成了一定影响。

Objective To study the reproductive injury of alcohol exposure on Caenorhabditis elegans （C.elegans） and the influence on exercise capacities of offspring. Methods L4 C.elegans were exposed to alcohol at 1%, 2%, 3%, 4%, 5%. We determined the numbers of offspring, eggs in uterus, ooeytes by using microscope. Then also calculated the ovulation rate of parental and observed the exercise capacities of offspring. Results Compared with the group of con- trol, the numbers of offspring, eggs in uterus, oocytes of C.elegans exposed to alcohol at 2%, 3%, 4%, 5% were de- creased obviously as well as the ovulation rates exposed at 3%, 4%, 5% （P 〈 0.05）. The exercise capacities of all the offspring were inhibited. Conclusion After a long-term exposure to alcohol, the reproductive capacity of C.elegans is damaged and this kind of injury might be reflected from the decline of the exercise capacities of offspring.