薯莨中鞣质纯化工艺优选

Optimization of Purification Technology of Tannin in Diascorea cirrhosa

目的:优选薯莨中鞣质的纯化工艺条件。方法:以鞣质含量和保留率为指标,采用静态吸附法考察不同大孔树脂的吸附、洗脱性能,通过单因素试验和L9(34)正交试验对薯莨中鞣质的纯化工艺条件进行优化。结果:选用AB-8型大孔吸附树脂,其最佳纯化工艺条件为上样量以每克树脂儿茶素不超过17.25 mg为宜,加3 BV水洗除杂,用1.5 BV 40%乙醇以0.5BV.h-1洗脱,收集洗脱液,鞣质保留率82.02%,鞣质纯度64.39%。结论:优选的纯化工艺合理、稳定可行,可为薯莨中鞣质的工业化纯化提供实验依据。

Objective： To optimize purification technology conditions of tannin from Diascorea cirrhosa. Method： With the content and retention rate of tannin as indexes, different macroporous resin properties of absorption and desorption were observed by static absorption method, purification technology conditions of tannin from D. cirrhosa was optimized by single factor test and L9 （34） orthgonal design. Result： AB-8 type macroporous resin was chosen, its optimum purification technology conditions were as followings： sample amount no more than 17.25 mg per gram of resin, washed ipurity with 3 BV water, and eluted by 1.5 BV 40% ethanol with flow rate of 0.5 BV·h-1, collected the eluent. The content and retention rate of tannin were 82.02% , 64. 39%, respectively. Conclusion： This optimized technology was stable, feasible and reasonable, it could provide experimental basis for industrial purification of tannin in D. cirrhosa.