星点设计效应面法优化染料木苷的酶解工艺

Optimization of Enzymatic Hydrolysis Technology of Genistin by Central Composite Design-Response Surface Methodology

目的:优选染料木苷的酶解工艺。方法:利用超微粉碎技术过筛制备不同粒径的染料木苷原料,采用纤维素酶将其水解,制备染料木素。以染料木素转化率为指标,通过单因素试验考察粉酶解时间、温度、粒径及pH对酶解工艺的影响,响应面法优化染料木苷超微粉酶解制备染料木素的工艺。结果:染料木苷超微粉酶解反应最佳条件为反应介质pH 5.5乙酸-乙酸钠缓冲液,温度49℃,反应时间11 h,酶与底物比1∶1。转化率(96.47±1.12)%,比普通粉染料木苷转化率高了1.84倍。结论:采用纤维素酶水解染料木苷超微粉制备染料木素的方法简单、酶解时间显著缩短,适用于规模化生产。

Objective： To optimize enzymatic hydrolysis technology of genistin. Method： Materials of genistin with different size were prepared by uhrafine grinding technology, then genistin was hydrolysised by celluse to get genistein. With the conversion rate of genistein as index, single factor tests were used to investigate effects of enzymatic hydrolysis time, temperature, size and pH on enzymatic hydrosis technology, and then, preparation technology of genistein was optimize by central composite design response surface methodology, which was enzymatic from genistin ultrafine powder. Result： Optimum technology conditions to get genistein through enzymatic hydrolysis genistin uhrafine powder were as followings： pH 5.5 acetic acid and sodium acetate anhydrous buffer as reaction medium, temperature 49℃, reaction time 11 h and ratio of substrate-enzymel ： 1. Under these conditions, the conversion rate of genistein was （96.47±1.12 ）% , which comparing with genistin ordinary powder, the conversion rate increased 1.84 folds. Conclusion： This method of enzymatic hydrolysis genistin ultrafine powder by eelluse to obtain genistein was simple, efficiency and suitable for modem large-scale production.