天钩降压胶囊中钩藤碱、异钩藤碱在大鼠离体小肠的吸收动力学研究

Studies on Absorption Kinetics of Rhynchophylline and Isorhynchophylline of Tiangou Jiangya Capsules in Rats' Intestinal

目的:研究天钩降压胶囊中有效成分钩藤碱、异钩藤碱的大鼠离体小肠吸收动力学。方法:采用外翻肠囊法,用HPLC测定不同取样时间肠内液钩藤碱、异钩藤碱的含量并计算累计吸收量,以钩藤碱、异钩藤碱的累计吸收量对时间作线性回归分析,计算吸收动力学参数。结果:钩藤碱回归方程为Y=0.000 9X-0.008 5(r=0.908 6),Ka=9.0×10-4(μg.min-1.cm-2),t1/2=12.8 h,异钩藤碱回归方程为Y=0.008 6X-0.178 2(r=0.909 0),Ka=8.6×10-3(μg.min-1.cm-2),t1/2=1.34h。结论:天钩降压胶囊中钩藤碱、异钩藤碱在大鼠离体小肠的吸收为一级动力学过程。

Objective： To study the absorption kinetics of active components which was rhynchophylline and isorhynchophylline of Tiangou Jiangya capsules in rat＇s intestinal. Method： Using an improved rat everted gut sac model, rhynchophylline concentration and isorhynchophylline concentration were determined by HPLC. The concentration of cumulative absorption amount was calculated. The linear regression equation was figured through the concentration of cumulative absorption amount and time. The parameters were calculated. Result： The regression equation of rhynchophylline was Y=0. 000 9X -0. 008 5 （r =0. 908 6） , K, =9.0×10^-4μg -min-1 -2 cm , t1/2 = 12.8 h ; the regression equation of isorhynchophylline is Y = 0. 008 6X - 0. 178 2 （ r = 0. 909 0）, K4= 8.6×10-3 μg·mm-1 cm-2 , t1/2 = 1.34 h. Conclusion： The absorption of rhynchophylline and isorhyncho- phylline conforms to the first-order kinetics in rats＇ intestine.