甘草酸与乌头碱配伍对神经细胞的作用

Effect of the Compalibility of Glycyrrhizic Acid and Aconitine on Nerve Cell

目的:研究甘草酸与乌头碱配伍对神经细胞的作用。方法:利用细胞培养技术体外培养大鼠大脑皮质神经细胞,运用MTT法测定甘草酸与乌头碱配伍对神经细胞存活率的影响,采用比色法测定甘草酸与乌头碱配伍对神经细胞内Na+,K+含量,钠-钾三磷酸腺苷酶(Na+,K+-ATPase)活力的影响。结果:1,2,4 g.L-1的甘草酸分别与2 g.L-1乌头碱配伍作用神经细胞30 min后,能不同程度拮抗乌头碱所致活细胞数降低,以及拮抗乌头碱所引起的神经细胞内Na+含量降低,K+含量和Na+,K+A-TPase活力的升高。结论:甘草酸能降低乌头碱所致神经细胞毒性,拮抗乌头碱所引起的神经细胞内环境的紊乱,乌头碱与甘草酸以1∶2配伍为佳。

Objective： To explore the effect of the compalibility of glycyrrhizic acid and aconitine on nerve cell. Method： Neuron cells were prepared from neonatal SD rats and cultured in vitro. The MTT assay was used to determine how mixture of the glycyrrhizic acid and aconitine in different proportions infect the survival of nerve cell. Then the activity of Na ＋ , K＋-ATPase and the contents of [ Na ＋ ], [ K＋ ] in the cells were measured using colorimetric method. Result： The activity of Na ＋ , K＋ -ATPase and the contents of [ K ＋ ] in the cells were increased, while the contents of [ Na＋ ] in the cells and the survival rate of nerve cell were decreased, after administration by the 2 g·L-1 aconitine for 30 min. The mixture of glycyrrhizic acid and aconitine in different proportions could lighten those changes caused by aconitine. Conclusion： Glycyrrhizic acid probably can decrease the toxicity of aconitine and antagonise the disturbance of internal environment in nerve cells. Besides, the best compatibility proportion is 1：2.