摘要
目的:通过探讨参附注射液对新生大鼠心力衰竭心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活性及细胞内Na+,Mg2+,K+,Ca2+浓度的影响,揭示参附注射液强心作用的机制。方法:通过胰蛋白酶消化法和差速贴壁法获得心肌细胞,经0.8%戊巴比妥钠作用5min后,分别给予1.5,3,6 mg.L-1(5,10,20 mL.L-1)3个不同浓度的参附注射液,作用1 h,检测心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶,Na+-K+-ATP酶的活性和细胞内Na+,Mg2+,K+,Ca2+的浓度。结果:参附注射液能增加心衰模型心肌细胞的搏动强度,提高Ca2+-ATP酶和Ca2+-Mg2+-ATP酶的活性,抑制Na+-K+-ATP酶的活性,降低细胞内K+,Ca2+的浓度,升高细胞内Na+,Mg2+的浓度。结论:参附注射液对戊巴比妥钠致心衰细胞有明显的保护作用,且作用的发挥与调节心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶,Na+-K+-ATP酶的活性和细胞内Na+,Mg2+,K+,Ca2+的浓度有关。
Objective: To study the effects of Shenfu injection on the Ca2+-Mg2+-ATP ase, Ca2+ -ATP ase, Na+-K+-ATP and Na+, Mg2+, K+, Ca2. in the myocardial cell injured by nembutal. Method: The myocardial cell is gotten by trypsin digestion and purified by different velocity of adherence. After injured by 0.8% nembutal, the myocardial cell is administrated with 3 different concentrations of Shenfu Injection. Then mensurate the vitality of the Ca2+ -Mg2 + -ATP ase, Na+ -K+ -ATP ase and Ca2+ -ATP ase and Na+, Mg2+ , K+, Ca2+ in the cell 1 h later. Result: The Shenfu injection can increase the strength of the myocardial cell beating injured by nembutal, and enhance the activity of Ca2+ -ATP ase and the Ca2. -Mg2+ -ATP ase, inhibit the the activity of Na+ - K + -ATP, enzyme increase the content of Na+, Mg+ and decrease the content of K + , Ca2+ in the myocardial cell. Conclusion: The Shenfu injection can protect the myocardial cell injured by nembutal, and the function is relevent to the effects of Shenfu injection on the Ca2+ -Mg2+ -ATP ase, Na+ -K + -ATP ase, Ca2+ -ATP ase and the content of Na+, Mg2+, K+, Ca2+ of myocardial cell.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第7期196-199,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十二五"科技支撑计划(2011BA113B05)
国家科技重大专项--重大新药创制专项(2013ZX09201018)