参附注射液对戊巴比妥钠致心衰模型心肌细胞膜ATP酶和相关离子的影响

Effects of the Shenfu Injection on ATPase and Related Ion of Heart Failure Model Myocardial Cell

目的:通过探讨参附注射液对新生大鼠心力衰竭心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶、Na+-K+-ATP酶活性及细胞内Na+,Mg2+,K+,Ca2+浓度的影响,揭示参附注射液强心作用的机制。方法:通过胰蛋白酶消化法和差速贴壁法获得心肌细胞,经0.8%戊巴比妥钠作用5min后,分别给予1.5,3,6 mg.L-1(5,10,20 mL.L-1)3个不同浓度的参附注射液,作用1 h,检测心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶,Na+-K+-ATP酶的活性和细胞内Na+,Mg2+,K+,Ca2+的浓度。结果:参附注射液能增加心衰模型心肌细胞的搏动强度,提高Ca2+-ATP酶和Ca2+-Mg2+-ATP酶的活性,抑制Na+-K+-ATP酶的活性,降低细胞内K+,Ca2+的浓度,升高细胞内Na+,Mg2+的浓度。结论:参附注射液对戊巴比妥钠致心衰细胞有明显的保护作用,且作用的发挥与调节心肌细胞Ca2+-ATP酶,Ca2+-Mg2+-ATP酶,Na+-K+-ATP酶的活性和细胞内Na+,Mg2+,K+,Ca2+的浓度有关。

Objective： To study the effects of Shenfu injection on the Ca2＋-Mg2＋-ATP ase, Ca2＋ -ATP ase, Na＋-K＋-ATP and Na＋, Mg2＋, K＋, Ca2. in the myocardial cell injured by nembutal. Method： The myocardial cell is gotten by trypsin digestion and purified by different velocity of adherence. After injured by 0.8% nembutal, the myocardial cell is administrated with 3 different concentrations of Shenfu Injection. Then mensurate the vitality of the Ca2＋ -Mg2 ＋ -ATP ase, Na＋ -K＋ -ATP ase and Ca2＋ -ATP ase and Na＋, Mg2＋ , K＋, Ca2＋ in the cell 1 h later. Result： The Shenfu injection can increase the strength of the myocardial cell beating injured by nembutal, and enhance the activity of Ca2＋ -ATP ase and the Ca2. -Mg2＋ -ATP ase, inhibit the the activity of Na＋ - K ＋ -ATP, enzyme increase the content of Na＋, Mg＋ and decrease the content of K ＋ , Ca2＋ in the myocardial cell. Conclusion： The Shenfu injection can protect the myocardial cell injured by nembutal, and the function is relevent to the effects of Shenfu injection on the Ca2＋ -Mg2＋ -ATP ase, Na＋ -K ＋ -ATP ase, Ca2＋ -ATP ase and the content of Na＋, Mg2＋, K＋, Ca2＋ of myocardial cell.