紫茉莉根醇提物对实验性2型糖尿病大鼠糖脂代谢的影响

Effects of Ethanolic Extract of Mirabilis jalapa Roots on Glucolipid Metabolization in Diabetic Rats

目的:观察紫茉莉根醇提物对实验性2型糖尿病大鼠血糖、血脂及胰岛素水平的影响。方法:注射链脲佐菌素(STZ,40 mg.kg-1,ip)加高糖高脂饲料喂养建立2型糖尿病大鼠模型,大鼠分为正常组、模型组、低中高剂量紫茉莉根醇提物(1,2,4 g.kg-1)和罗格列酮组(4 mg.kg-1),连续给药8周。给药后每2周测1次空腹血糖(FBG),试剂盒检测糖化血红蛋白(HbAlC)、总胆固醇(TC)、甘油三酯(TG)、肝糖原及肌糖原含量,放射免疫法测血清胰岛素(FINS)。结果:给药8周后,糖尿病模型组大鼠FBG,HbAlC,TC,TG,FINS显著升高,胰岛素敏感指数(ISI)、肝糖原、肌糖原水平均显著下降(P<0.01);与糖尿病模型组相比,紫茉莉根醇提物中、高剂量组大鼠FBG,HbAlC,TC,TG,FINS水平下降,ISI,肝糖原及肌糖原水平均上升,均有显著差异(P<0.01)。结论:紫茉莉根醇提物对实验性2型糖尿病大鼠具降糖调脂作用。

Objective： To investigate the effects of ethanolic extract of Mirabilis jalapa L. roots on glucolipid metabolization in diabetic rats. Method： Type 2 diabetes mellitus rats were induced by intraperitoneal injection 40 mg·kg-1 streptozotocin and a high-carbohydrate/high-fat diet. Rats were divided into following groups： normal group, model group, ethanolic extract of M. jalapa roots group （1, 2, 4 g-kg-1） and rosiglitazone group （4 mg .kg-1 ）. After 8-weeks treatment, fasting blood glucose （FBG） , hemoglobin （HbA1C） , total cholesterol （TC）, triglyceride （TG）, serum insulin （FINS） levels and glycogen contents in liver and skeletal muscle were assayed. Result： Compared with the normal group, FBG, HbA1C, TC, TG, FINS levels were significantly increased, and insulin sensitivity index （ISI）, glycogen contents in liver and skeletal muscle decreased significantly in the model group （P 〈0.01）. Compared with the model group, the levels of FBG, HbA1C, TC, TG, FINS decreased significantly, and ISI, glycogen contents in liver and skeletal muscle increased significantly in the ethanolic extract of M. jalapa roots group （2, 4 g·kg- 1 ） （p 〈 0.01 ）. Conclusion： ethanolic extract of M. jalapa roots possesses both potential hypoglycemic and hypolipidemic effects on diabetes.