野生与家养五灵脂药效学比较研究

Pharmacodynamic Comparison between Wild and Breeding Faeces Trogopterori

目的:对野生和家养复齿鼯鼠所产五灵脂的活血化瘀及抗炎作用进行比较。方法:抗炎实验动物随机分为8组:模型对照组,阳性药组(阿司匹林0.2 g.kg-1),家养(陕西)五灵脂高、中、低剂量组(生药9,4.5,2.25 g.kg-1),野生五灵脂高、中、低剂量组(生药9,4.5,2.25 g.kg-1),连续口服7 d;活血化瘀实验动物分为9组:空白对照组、模型组,阳性药组(阿司匹林0.05 g.kg-1)及6个给药组(剂量及疗程同前)。给药后以角叉菜胶致大鼠足肿胀模型,观测30 min,1,2,4,6,24 h足跖肿胀度;利用大鼠血瘀证模型,检测不同切变率下的全血黏度、红细胞压积、血浆黏度、血小板聚集率和血浆纤维蛋白原含量。结果:各给药组在给药后1 h作用最强,家养五灵脂作用时间持久,高剂量在6 h仍可以明显降低足跖肿胀度[由(0.141±0.073)mL降至(0.075±0.025)mL]。家养五灵脂对血液流变学指标没有明显影响,野生五灵脂可以降低全血黏度。结论:家养五灵脂抗炎作用优于野生五灵脂,但没有显示出活血化瘀作用。

Objective： To observe the effects between Wild and Breeding double teeth mole Faeces Trogopterori on removing blood stasis, anti-inflammatory in rat with acute blood stasis syndrome and paw edema inflammation. Method： Rats were randomly divided into 8 groups for anti-inflammation test： model control group, positive medicine group （aspirin 0.2 g·kg-1）, shannxi Faeces Trogopterori of higher, middle and low dosage groups （9, 4.5, 2.25 g·kg-1） , wild Faeces Trogopterori of higher, middle and low dosage groups （9, 4.5,2.25 g·kg-1）. All groups were treated continuously for 7 days. Rats were randomly divided into 9 groups for activating- blood： normal control group, model control group, positive group （aspirin 0.05 g·kg-1） and 6 treatment groups which use the same dose and time to the anti-inflammation test. After treatment, carrageenan was injected subcutaneously in the pad of the rat＇s hindfoot. The volume of the rat＇s hindfoot was measured at 30 rain, 1, 2, 4 h, and 6 h respectively after the modeling, and the degrees of swelling （E%）. The indexes of hemorheology including whole blood viscosity under different shear rates, packed cell volume, plasma viscosity, platelet aggregation and fibrinogen were detected for the rat blood stasis syndrome model. Result： All the treatment groups showed the strongest effect after treatment for 1 h. Faeces Trogopterori from Shannxi had the lasting action time. Higher dosage still showed, an obviously decreasing digits swelling degree after 6 h. There was no significant effect of from shaanxi on blood rheology indexes, and whole blood viscosity could be decreased by wild Faeces Trogopterori. Conclusion： The effect of breeding Faeces Trogopterori on anti-inflammatory was better than the wild, but there was no significant effect on removing blood stasis.