天冬氨酸-谷氨酸共聚物的生物降解性及毒性研究

Biodegradability and toxicity of aspartic acid-glumic acid copolymer

目的探讨聚天冬氨酸新型改性产品天冬氨酸-谷氨酸共聚物(PAG)的生物降解性及毒性。方法取受纳水体的水为接种水,采用CO2法对浓度为98.8 mg/L PAG的可生物降解性进行研究;观察PAG灌胃染毒对SPF级昆明小鼠的急性毒性和骨髓嗜多染红细胞微核率;以5、1、0.2、0.04和0.008 mg/皿的剂量进行PAG对TA97、TA98、TA100、TA102菌株的Ames试验;观察100、500和1 000 mg/L浓度PAG对小麦及大豆种子发芽率的影响。结果 PAG的10 d和28 d生物降解率分别为34%和79%,属于易生物降解物质;小鼠经口半致死量(LD50)大于10 000 mg/kg;Ames试验结果呈阴性;PAG对植物生长发育无明显不良影响。结论 PAG为实际无毒且非致突变性的安全化学品。

Objective To explore the biodegradability and toxicity of aspartic acid-glutamic acid eopolymer （PAG）. Methods Surface water sample was collected as inoculated water, methods of CO2 test was carried out for the biodegradability. Acute toxicity test and micronucleus test on mouse hone marrow polychromatic erythrocytes for PAG（98.8 mg/L） were carried out by gavage.PAG was dissolved in DMSO （5, 1, 0.2, 0.04和 0.008 mg per plate） and negative and positive control group were designed for Ames test. Impact of PAG on germinating percentage was discussed. Results The 10-day and 28-day biodegradation rates of PAG were 34% and 79% and it was easily biodegradable substance. The oral LD50 of PAG for mice was higher than 10 000 mg/kg. The mutagenieity test result was negative. PAG had no significant adverse effects on plant growth and development. Conclusion PAG is an actually nontoxic and non-mutagenic chemical.