(乙醇＋丙酮)-硫酸铵二元双水相分离萃取盐酸四环素

Ethanol and acetone-ammonium sulfate binary aqueous two-phase extraction separation of tetracycline

基于小分子醇双水相体系和小分子有机物双水相体系,建立了(乙醇+丙酮)-(NH4)2SO4形成的二元双水相体系萃取盐酸四环素的新方法.研究了分相盐、醇及小分子有机物的类型、用量及比例、温度、溶液酸度、共存物质对盐酸四环素分配行为的影响.结果表明,硫酸铵浓度为41%、无水乙醇与丙酮体积比为2∶1、溶液酸度在pH 4.5—5范围、温度为25℃二元双水相体系对四环素有较高萃取率可达90%,分配系数达到57.当测试允许相对误差为±7%时,浓度为75 mg.L-1的盐酸四环素,K+、Na+、F-、Cl-、NO3-、Br-浓度均为10%对测定结果无干扰,1.5倍的盐酸美他环素、35倍的氯霉素、青霉素和红霉素、80倍的淀粉和蔗糖、400倍的葡萄糖和果糖对测定结果不发生干扰.

Based on small molecule alcohol and organic molecule aqueous two-phase system,a new method was developed for extraction of tetracycline hydrochloride in ethanol-acetone-ammonium sulfate binary aqueous system.Several factors on the tetracycline hydrochloride allocation behavior was studied,including the type,amounts and relative proportions of salt-alcohol-small molecular organics,temperature,pH and the coexisting substances.The results showed that when ammonium sulfate was 41%,the volume ratio of absolute ethyl alcohol and acetone was 2 ∶1,and the solution pH was 4.5—5,the extraction rate and the distribution coefficient of tetracycline hydrochloride were 90% and 57 respectively.The relative error was ±7%,the ions including K＋,Na＋,F-,Cl-,NO-3,Br-,the hydrochloride metacycline,and other components in the system such as chloramphenicol penicillin,erythromycin,starch and sucrose,glucose and fructose have not any effect on the extraction behavior.