阿司匹林对体外培养人宫颈癌细胞凋亡作用的研究

Effect of aspirin on apoptosis of human cervical cancer cell line in vitro

目的探讨阿司匹林诱导宫颈癌Hela细胞凋亡及对凋亡相关基因Bcl-2和bax的作用。方法培养人宫颈癌Hela细胞,加入不同浓度阿司匹林(1.0、5.0、10.0mmol/L)干预培养。应用流式细胞技术检测Hela细胞凋亡和细胞周期的变化;用RT-PCR检测细胞凋亡相关基因bcl-2及bax转录水平的改变;免疫组化法检测bcl-2及bax蛋白表达的变化。结果与对照组相比,阿司匹林干预组Hela细胞凋亡率明显增高(P<0.05);随着阿司匹林浓度增加,G0/G1期细胞比例下降,G2/M期细胞比例升高(P<0.05);阿司匹林干预培养后Hela细胞中bcl-2基因mRNA表达明显降低,而bax基因mRNA表达升高明显,较对照组均有统计学意义(P<0.05);阿司匹林作用48h后,免疫组化法检测bcl-2蛋白表达下调及bax蛋白表达上调(P<0.05)。结论阿司匹林能诱导宫颈癌Hela细胞凋亡,其机制与抑制bcl-2表达,上调bax表达有关。

Objective To investigate the effect of aspirin on apoptosis of human cervical cancer cell line（Hela） and expression of Bcl-2 and bax genes.Methods The Hela cells were cultured and induced by aspirin in different concentrations（1.0,5.0,10.0 mmol/L）.The effect of aspirin on apoptosis of Hela cell and the changes of cell cycle were observed by flow cytometry（FCM）.The levels of bcl-2 and bax mRNA were detected by RT-PC.The expressions of bcl-2 and bax in Hela cell line were detected by immuneohistochemistry.Results As compared with that in control group,the apoptosis rate of Hela cells treated with different doses of aspirin was significantly increased（P0.05）.The Hela cells at G0/G1 stage were decreased,however,which at G2/M stage were increased（P0.01）.The expression of bcl-2 and bax mRNA in Hela cells treated with different doses of aspirin was significantly decreased,however,the expression of bax mRNA was significantly increased,as compared with those in control group（P0.05）.After treated by aspirin for 48h,the expression of bcl-2 protein was down-regulated,but the expression of bax protein was up-regulated（P0.05）.Conclusion The aspirin can induce apoptosis of Hela cells,and its action mechanism is correlated with inhibition of bcl-2 expression and up-regulating expression of bax.