结肠癌组织AQP1与MMP-2、MMP-9、TIMP-1表达的关系及意义

Significance of expressions of AQP1,MMP-2,MMP-9,TIMP-1 in colon cancer and the correlation among them

目的检测水通道蛋白1(AQP1)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制因子-1(TIMP-1)在结肠癌组织中的表达,并分析这些蛋白质之间的关系,探讨其意义。方法对54例结肠癌组织和40例癌旁组织石蜡标本进行免疫组化染色,检测AQP1与ICAM-1、MMP-2、MMP-9、TIMP-1在两种组织中的表达情况。结果结肠癌组织中AQP1与ICAM-1、MMP-2、MMP-9、TIMP-1阳性表达率分别为75.93%、64.81%、77.78%、46.30%,而癌旁组织中阳性率则分别为22.50%、37.50%、50.00%、70.00%。AQP1、MMP-2、MMP-9在肿瘤组织中表达强度高于癌旁组织,而TIMP-1在结肠癌组织中表达则低于癌旁组织(P<0.05)。肿瘤组织中AQP1与MMP-2、MMP-9表达呈正相关性(r值分别为0.4030,0.3874,P<0.05),MMP-2、MMP-9与TIMP-1表达呈负相关(r值分别为-0.3176、-0.4728,P<0.05)。结论结肠癌组织中AQP1表达增强,AQP1可能通过调节MMP-2、MMP-9表达而促进结肠癌的侵袭转移。

Objective To detect the expressions of aquaporin-1（AQP1） and matrix metalloproteinase（MMP）-2,MMP-9,tissue inhibitor of matrixmetalloproteinase-1（TIMP-1） in colon cancer,and to explore their correlation.Methods The expressions of AQP1,MMP-2,MMP-9,TIMP-1 were detected by immunohistochemstry（SP） in 54 cases of colon cancer tissues and 40 cases of tumor-adjacent tissues.Results The positive expression rates of AQP1,MMP-2,MMP-9 in tumor tissues were significantly higher than those in tumor-adjacent tissues,but the positive expression rate of TIMP-1 in tumor tissue was obviously lower than that in tumor-adjacent tissues（P0.05）.The expression of AQP1 was positively correlated with that of MMP-2,MMP-9 in tumor tissues（P0.05）,however,the expression of MMP-2,MMP-9 was negatively correlated with that of TIMP-1（P0.05）.Conclusion AQP1 is over-expressed in colon cancer tissues,and AQP1 may promote invasion and metastasis of colon cancer by regulating the expression of MMP-2 and MMP-9.