戊胺和苯胺废水污染的微生物PN1001修复(英文)

Microbial PN1001 Remediation of Pentyl Amine and Anilin from Wastewater Pollution

利用从混合生物脱氮系统活性污泥中分离出的菌株PN1001,对含戊胺和苯胺的石化炼油废水污染进行生物修复.研究表明菌株PN1001对含戊胺和苯胺的石化炼油废水污染有很强的修复力.通过富集培养驯化后处理含戊胺和苯胺废水的菌株为假单胞菌.该菌株接种在复合生物脱氮系统反应器中的最佳实验操作条件为:温度为30℃,pH为7,水力停留时间(HRT)为24 h,正常曝气.在污水质量浓度小于210 mg/L时,戊胺的降解率达到86%;当污水质量浓度为130 mg/L时,苯胺降解率可达到93%.通过高效液相色谱分析苯胺生物降解的反应途径.研究结果为进一步修复含戊胺和苯胺污染的废水及土壤的处理奠定了基础.

A strain called PN1001 was isolated from the activated sludge of a hybrid bio-denitrification system（HBDS） treating by CBDS refinery petrochemical wastewater containing pentyl amine and aniline. Demonstrating a strong affinity for petrochemical compounds,this strain identified as Pseudomonas sp was acclimatized using an enrichment culture technique and wastewater containing pentyl amine and aniline. This strain was then used to inoculated a CBDS reactor and the optimal operating conditions were defined as：30 ℃ ,pH of 7.0,hydraulic retention time（HRT） of 24 h and normal aeration. Up to 86 % of pentyl amine was degraded when fed at a concentration of less than 210 mg/L. At concentrations under 130 mg/L ,93 % of the aniline was degraded. The research followed the reaction pathway for aniline biodegradation using HPLC and IC analysis. This project constitutes the foundation for further research on the treatment of wastewater and soil contaminated with petroleum compounds.