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牛病毒性腹泻病二价弱毒活疫苗制备工艺研究 被引量:3

Study on Preparation Technique of Divalent Attenuated Virus Live Vaccine of Bovine Viral Diarrhea Virus
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摘要 研究了BVDV2/JZ05-1和BVDV1/JZ05-3二价弱毒活疫苗的制备工艺。通过对BVDV2/JZ05-1和BVDV1/JZ05-3疫苗毒传代培养测定毒价后,试制出5批毒价分别是105.4TCID50/mL和105.6TCID50/mL的BVDV二价弱毒活疫苗,经检验符合试验设计要求。 The aim of this study was to explore the preparation technique of divalent attenuated virus live vaccine of bovine viral diarrhea virus 2/JZ05- 1 (BVDV2/JZ05- 1) and BVDV1/JZ05- 3. BVDV2/JZ05- 1 and BVDV1/JZ05- 3 were continuously cultivated in cell culture. The poison titor was examined.5 groups of divalent attenuated BVDV live vaccine were produced. The poison prices were 10^(5.4)TCID50/mL and 10^(5.6)TCID50/mL,respectively. The vaccines were examined and was within the range of the test design requirement.
出处 《特产研究》 2013年第1期1-2,18,共3页 Special Wild Economic Animal and Plant Research
基金 吉林省科技发展计划(20100219)
关键词 牛病毒性腹泻 病毒 活疫苗 bovine viral diarrhea virus live vaccine
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参考文献7

  • 1Reichel iMP, Hill FI, Voges H. Does Control of Bovine Vviral Di- an'hoea Infection Make Economic Ssense? [ J ]. New Zealand Vet J,2008,56(2) :60 - 66.
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二级参考文献9

  • 1ReichelMP, Hill FI, Voges H. Does control of bovine viral diarrhoea infection make economic sense? [J]. New Zealand Veterinary Journal, 2008, 56(2):60-66.
  • 2Olafson P, MacCallum AD, Fox FH. An apparently new trans-missible disease of cattle[J]. Comer Vet, 1946, 36:205-213.
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  • 4Luzzago C, Bandi C, Bronzo V, et al. Distribution pattern of bovine viral diarrhea virus strains in intensive cattle herds in Italy[J]. Vet Microbiolgy, 2001, 83(3):265-274.
  • 5Ridpath JF. Practical significance of heterogeneity among BVDV strains: impact of biotype and genotype on U.S. control programs[J]. Prey Vet Med, 2005, 72(1-2):17-30.
  • 6Reichel MP, Hill FI, Voges H. Does control of bovine viral diar- rhea infection make economic sense? [J]. New Zealand Veteri- nary Journal,2008,56(2) :60 - 66.
  • 7李庆超.牛病毒性腹泻病毒Jz05-l株基因分型、全基因序列测定及分析[D].北京:中国农业科学院特产研究所,2010.
  • 8李海涛,苗利光,刘艳环,王松,王文昊,安亚雄,冯卓.牛病毒性腹泻病活疫苗BVDV2/JZ05-1株毒力返强试验[J].特产研究,2012,34(1):6-7. 被引量:7
  • 9李海涛,苗利光,刘艳环,朱言柱,王松,王文昊,安亚雄.牛病毒性腹泻BVDV2/JZ05-1基础毒株的安全性和免疫原性研究[J].特产研究,2012,34(2):5-8. 被引量:2

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