食管鳞癌中Livin及相关蛋白的表达及意义

Expression and significance of Livin Ki-67 and Livin mRNA in esophageal squamous cell carcinoma

目的探讨食管鳞癌组织中Livin、Ki-67蛋白和Livin mRNA的表达及其与临床病理指标和预后的关系。方法采用原位杂交方法和微波EliVisionTM免疫组织化学法检测78例食管鳞癌组织中Livin、Ki-67蛋白和Livin mRNA蛋白的表达情况,并与38例癌旁正常食管黏膜组织进行比较。结果在食管鳞癌组织中Livin和Ki-67分别表达定位于细胞浆和细胞核,Livin mRNA阳性反应主要定位于细胞浆,其表达明显高于正常食管黏膜组织(P<0.05)。Livin、Ki-67蛋白和Livin mRNA的表达与淋巴结转移、分化程度及病理分期有关(P均<0.05),三者之间表达呈正相关(P均<0.05)。结论 Livin、Ki-67蛋白和Livin mRNA在食管鳞癌呈高表达,且与食管鳞癌的发生、发展及浸润转移有关,可作为判断食管鳞癌生物学行为和预后的重要指标。

Objective It is to explore the expression of Livin, Ki -67 and Livin mRNA in esophageal squamous cell carcinoma and its relationship with clinical pathologic indexes and prognosis. Methods The expressions of Livin, Ki - 67 and Livin mRNA in 78 cases of esophageal squamous cell carcinoma were detected by in-situ hybridization method and microwave EliVisionTM, and they were compared with that of 40 cases of normal endometrial tissue. Results The expression of Livin and Ki - 67 located in the nucleus and plasma cells. The expression of Livin mRNA located in the nucleus. Its expression was higher than that of the normal tissue（ P 〈 0.05 ）. All of them were related with differentiation degree, lymph node metastasis and path- ological staging（ P 〈 0.05 ）. There was positive correlation among them （ P 〈 0.05 ）. Conclusion Livin, Ki - 67 and Livin mRNA were highly expressed in the esophageal squamous cell carcinoma and the expressions were related with esophageal squamous cell carcinoma development and metastasis. It can be used as important indexes for diagnosing biological actions and prognosis of esophageal squamous cell carcinoma.