远志皂苷元对AD模型小鼠学习记忆能力的影响及其机制研究

Effect of Senegenin on learning and memory ability of AD model mice and its mechanism

目的观察远志皂苷元对阿尔茨海默病(AD)模型小鼠的学习记忆能力的影响,并初步探讨其作用机制。方法采用对小鼠海马立体定向注射Aβ1-42的方法建立AD动物模型,并随机分为假手术组,模型组,低剂量、中剂量、高剂量治疗组,连续治疗30 d后,运用Morris水迷宫检测各组小鼠学习记忆能力,随后进行活性氧簇(ROS)、8-羟基脱氧鸟苷(8-OHdG)、3-硝基酪氨酸(3-NT)等氧化应激指标检测。结果与模型组相比,各治疗组小鼠的潜伏期明显缩短,穿台次数明显增多,海马组织的ROS、8-OHdG、3-NT含量均有明显减少(P均<0.05)。结论远志皂苷元能明显改善AD模型小鼠的学习记忆能力,其机制可能与抗氧化应激作用有关。

Objective It is to observe the effect of Senegenin on learning and memory abilities of Alzheimer＇s disease （AD） model mice, and to explore its mechanism. Methods Using mouse hippocampus stereotactic injection of Aβ （1 -42） method to establish animal models of （AD） , and the models were randomly divided into sham group, model group, low-dose, me- dium-dose and high-dose treatment groups, after continuous treatment of 30 d using Morris water maze to test learning and memory abilities of the mice in each group, then oxidative stress indicators such as reactive oxygen clusters （ROS） , 8 - hydroxy-de- oxy-guanosine （8 -OHdG） , 3 -nitrotyrosine（3 -NT） were determined. Results Compared with model group, the incubation period of the mice in treatment groups was significantly decreased, wear station was significantly increased, and the contents of hippocampus ROS, 8 - OHdG, 3 - NT were significantly reduced （ all P 〈 0.05）. Conclusion Senegenin can significantly improve learning and memory abilities of AD model mice, the mechanism may be related to its effect of anti-oxidative stress.