摘要
对壳聚糖酶理化性质进行初步实验研究,通过测定不同条件下酶的活力来研究酶的性质.实验结果表明,所用菌种所产壳聚糖酶最适反应温度和pH值分别为50℃和5.6,NH+对该酶有明显的促进作用,而Na+,K+,Mg2+,Cu2+,Ag+,NH+,Mn2+对该酶有一定的抑制作用.Fe3+,Ca2+对酶的抵制作用最明显.
The physical and chemical properties of chitosanase were studied,by means of measuring the activity of this enzyme under various conditions.Results showed that the optimal temperature for chitosanase was 50 ℃ and the optimal pH was 5.6.NH ＋ enhanced the enzymic activity considerably,whereas Fe3＋,Ca2＋inhibited the activity to some degree.Na＋,K＋,Mg2＋,Cu2＋,Ag＋ and Mn2＋ have the most conspicuous inhibition of this enzyme.
出处
《甘肃科学学报》
2013年第1期31-34,共4页
Journal of Gansu Sciences
基金
广东省科技计划项目(2009B011300009)
关键词
壳聚糖酶
理化性质
酶活力
chitosanase
physical and chemical nature
enzymic activity
