参芪解郁方对产后抑郁大鼠脑组织ER表达分布的干预研究

Research of influence of Shenqijieyu prescription on expression and distribution of ER in brain tissue in rats with postpartum depression

目的探讨参芪解郁方对产后抑郁(PPD)大鼠脑内不同区域雌激素受体(ER)表达变化及分布规律的干预机制。方法选用雌性2～3月龄SD大鼠,随机分为空白、假手术、模型、中药、西药组,每组再设1、2、4、6周观察时点。采用免疫组化法测定脑前额皮质、海马、下丘脑ERα、ERβ表达。结果 ERα模型组第2、4、6周前额皮质、海马、下丘脑表达较空白、假手术组减少,差异有统计学意义(P<0.01,P<0.05),第1周海马、下丘脑表达较空白、假手术组增加,差异有统计学意义(P<0.01,P<0.05)。中药组第4、6周前额皮质,西药组第2、4、6周前额皮质、下丘脑,中、西药组第2、4、6周海马,中药组各时点下丘脑表达均较模型组增加,差异有统计学意义(P<0.01,P<0.05)。ERβ:模型组1周前额皮质,第1、2周海马、下丘脑表达均较空白、假手术组增加,差异有统计学意义(P<0.01,P<0.05),第4、6周前额皮质、下丘脑及第6周海马表达均较空白、假手术组减少,差异有统计学意义(P<0.01)。中、西药组第6周前额皮质及第4、6周海马,中药组第4、6周及西药组第2、4、6周下丘脑所有表达均较模型组增加,差异有统计学意义(P<0.01,P<0.05)。结论参芪解郁方能够明显促进PPD大鼠脑组织内ER尤其是ERβ的表达上调,对脑组织内不同区域ER表达的调节改善可能是该方作用发挥的重要途径之一。

Objective To explore interventional mechanism of Shenqijieyu prescription on postpartum depression （PPD） from the expression changes of estrogen receptor （ER） in different areas of brain and the distribution law. Methods Female 2-3 month SD rats were selected and randomly divided into a blank group, a sham-operated group, a model group, a Chinese medicinal group and a western medicine group. The time points at 1st, 2nd, 4th, 6th week were set for observation in each group. The expressions of ER ct, ER 13 in forebrain cortex, hippocampus and hypothalamus were detected by immunohistochemistry. Results For ER ot ,in the model group, the expressions of forebrain cortex, hippocampus and hypothalamus at 2nd, 4th, 6th week were decreased compared with those in the blank group and the sham-operated group with significant statistical differences （P 〈 0.01, P 〈 0.05）; while the expressions in hippocampus and hypothalamus at 1st week were increased （P 〈 0.01, P 〈 0.05）. Expressions in the forebrain cortex at 4th, 6th week in the Chinese medicinal group, forebrain cortex and hypothalamus at 2nd, 4th, 6th week in the western medicine group, hippocampus at 2nd, 4th, 6th week in both groups and hypothalamus at each time point in the Chinese medicinal group were increased compared with those in the model gwup with significant statistical differences （P 〈 0.01, P 〈 0.05）. As for ER 13, in the model group, expressions in the forebrain cortex at 1st week,as well as in hippocampus and hypothalamus at 1st, 2nd week were increased compared with those in the blank group and the sham-operated group with significant statistical differences （P 〈 0.01, P 〈 0.05）; while expressions in the forebrain cortex, hypothalamus at 4th, 6th week and expression in hippocampus at 6th week were decreased （P 〈 0.01）. The expressions in the forebrain cortex at 6th week, in hippocampus at 4th, 6th week in the Chinese group and the western group, as well as exporessions in the hypothalamus at 4th, 6th week in the Chinese group and at 2nd, 4th, 6th week in the western group were increased compared with those in the model group with significant statistical differences （P 〈 0.01, P 〈 0.05）. Conclusion The Shenqijieyu prescription could apparently promoting the expressions of ER, especially for ER 13, in brain tissues for PPD rats. The regulation and improvement on expressions of ER in different areas of brain could be one of important ways to exert its function.