临床分离142株洋葱伯克霍尔德菌分布特征及耐药性分析

Distribution and drug resistance of 142 strains of Burkholderia cepacia isolated from clinical specimens

目的了解南海区第三人民医院和佛山市第一人民医院洋葱伯克霍尔德菌医院感染的分布及耐药谱,为临床治疗及医院感染的控制提供依据。方法 2010年1月至2012年6月临床分离142株菌株,细菌鉴定和大多数药敏试验利用Vitek-compact全自动微生物分析仪,少数利用ATB Expression半自动微生物分析仪,个别药敏试验采用K-B法。药敏数据分析用WHONET5.4软件。结果痰液标本分离洋葱伯克霍尔德菌的阳性率最高,达到78.2%。洋葱伯克霍尔德菌对多种抗菌药物耐药,但对头孢吡肟、哌拉西林/他唑巴坦、左氧氟沙星、头孢他啶、复方磺胺甲噁唑敏感率较高,敏感率分别为83.1%、87.2%、87.3%、90.3%、90.9%。结论洋葱伯克霍尔德菌已成为医院分离的常见病原菌之一,医院应重视其耐药性监测,指导临床合理使用抗菌药物。

Objective To investigate the distribution and drug resistance of Burkholderia cepacia isolated from clinical specimens. Methods 142 strains were identified by Vitek-eompact. Antibiotics susceptivity tests were made by Vilek-compact or K B method. Drug resistance was analyzed by WHONETS. 4. Results The pathogenic bacteria were mainly isolated from sputum （78.20//oo）. High levels of resistance to most antibiotics were found in Burkhotderia cepacia isolates. The sensitive rates of Burkholderia cepaeia to Cefepime, Piperacillin/tazobactam, Levofloxacin, Ceftazidime and Trimethoprim/sulfamethoxazole were 83. 1%, 87.2 %,87.3%, 90.3% and 90. 9%, respectively. Conclusion Burkholderia cepacia is one of the common pathogenic bacteria from clinical specimens. Hospitals should pay attention 1o the root cause of drug resistant bacteria and rational use of antimicrobial agent.