中国土家族人群血管紧张素原和血管紧张素转化酶基因多态性与冠心病的关系

Association between the gene polymorphism of angiotensinogen and angiotensin converting enzyme and coronary artery disease

目的研究血管紧张素原(AGT)基因M235T分子变异和血管紧张素转化酶(ACE)基因I/D多态性与冠状动脉粥样硬化的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测冠心病(CHD)组137例和健康对照组125例AGT基因多态性,采用聚合酶链反应技术检测CHD组和健康对照组ACE基因I/D多态性。结果 CHD组AGT-TT基因型频率为75.91%,显著高于健康对照组43.20%(P<0.01);ACE-DD基因型频率为35.77%,显著高于健康对照组15.20%(P<0.01)。结论在中国土家族人群中,AGT基因TT基因型和ACE基因DD基因型是CHD发病既相互独立又具有协同作用的危险因子。

Objective To investigate the relation between the polymorphisms of angiotensinogen （ACT） gene- type M235T and angiotensin-converting enzyme （ACE） genetype I/D and coronary atherosclerosis. Methods The study population was consisted of 151 patients with coronary heart disease （CHD）, and the healthy control group was consisted of 127 patients without CHD. Patients with CHD were compared with those persons in the healthy control group. ACT gene polymorphisms was tested with polymerase chain reaction and restriction fragment length polymor- phism （PCR-RFLP） technology and ACE gene polymorphism was tested with PCR. Results The ratios of AGT_＇IT and ACE-DD genetype in CHD group were 75.91% and 43.20%, respectively, which were significantly higher than that of control group （35.77% and 15.20% ）. There was significant difference of the ACT_＇TT genetype between the CHD group and the control group （P〈0.01） ,so was ACE-DD genetype between the two group （P〈0.01）. Conclusion In the population of Chinese miao nationality, the gene polymorphisms of genetype AGT-TT and genetype ACE-DD are two risk factors of CHD morbidity and they are both independent of each other,but also mutually coordinated.