不同标本前处理方法对血管紧张素测定结果的影响

Effect of different specimens pretreatment on determination of angiotensin

[目的]探讨不同的标本前处理方法对血管紧张素Ⅰ、Ⅱ(AⅠ、AⅡ)测定结果的影响。[方法]分别采用3种不同方法对40例病人的静脉血标本进行处理,分离血浆后用化学发光法检测AⅠ和AⅡ的水平。方法 1为按说明书用血常规真空管采血后立即加入酶抑制剂;方法 2为在血常规管中事先加好酶抑制剂再采血;方法 3为用血常规管采血后在冰箱放置1 h再加入酶抑制剂。[结果]方法 2检测出的AⅠ、AⅡ水平与方法 1的检测结果差异无统计学意义,方法 3检测的AⅠ、AⅡ水平明显低于方法 1。[结论]加酶抑制剂能抑制AⅠ、AⅡ、AⅢ等转换,先加入酶抑制剂和采血后再加入对检测结果均无明显影响,方法 2还可避免漏加抑制剂。

[ Objective ] To discuss the effect of different specimens pretreatments on determination of angiotensin I and II levels. [ Methods ] A total of 40 blood samples were collected and used by three different treatment methods （ method 1 ： blood was drawn into EDTA - I（2 anticoagulated vacuum tube, then added with enzyme inhibitor immediately, method 2 ： enzyme inhibitor was added to the EDTA - K2 antico- agulated vacuum tube first, then mixed with blood, method 3 ： the enzyme inhibitor was added at one hour after blood drawn was placed in the refrigerator} , after separation of plasma, the levels of AI and All were detected by chemiluminescence. [ Results ] The results of AI and All by method 2 were not significantly different from those by the method 1. But the levels were significantly descended using method 3 as compared with method 1. [ Conclusion ] There were inhibitory effects of enzyme inhibitor on the conversion among AI ,All and Am. Adding enzyme inhibitor first makes no significant difference to detec- tion results from adding it after blood drawing and helps not to forget to add the inhibitor.