桂花幼胚的离体培养及生根诱导

Culture in vitro and Root Induction of Immature Embryos of Osmanthus fragrans

对3个桂花品种大花金桂、籽银桂、籽丹桂进行幼胚的离体培养,研究了不同消毒时间、基本培养基及生长调节剂组合对离体幼胚萌发的影响,以及不同培养条件和培养基配方对萌发苗生根的影响。结果表明,适合幼胚萌发的适宜培养基,大花金桂为:B5+1 mg/L 6-BA+3 mg/L GA;籽银桂:B5+2 mg/L 6-BA+2 mg/L GA;籽丹桂:B5+1.5 mg/L 6-BA+1 mg/L GA。生根适宜培养基为:1/2 MS+2 mg/L NAA。

Culture in vitro of immature embryos of three species of Osmanthus fragrans as ＇ Dahua Jin- gui＇ , ＇ Zi Yin＇ and ＇ Zi Dangui＇ were carried out. The effects of different sterilization time, basic culture me- dium and different growth regulator on in vitro embryo germination, as well as the different culture conditions and medium on root induction were focused on. The results indicated that the optimal media for embryo germi- nation of ＇ Dahua Jingui＇ ,＇ Zi Yin＇ and ＇ Zi Dangui＇ was B5 ＋ 1 mg/L 6 - BA ＋ 3 mg/L GA, Bs ＋ 2 mg/L 6 - BA ＋ 2 mg/L GA and B5 ＋ 1.5 mg/L 6 - BA ＋ 1 mg/L GA respectively . The suitable medium for rooting was 1/2MS ＋ 2 mg/L NAA.